Genome characterization of a novel chicken picornavirus distantly related to the members of genus Avihepatovirus with a single 2A protein and a megrivirus-like 3' UTR

Ákos Boros, P. Pankovics, Ádám Adonyi, Tung Gia Phan, Eric Delwart, G. Reuter

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The members of the genus Avihepatovirus and related picornaviruses ("Aalivius") of ducks, turkey and chickens possess identical 2A peptide composition including three functionally unrelated 2A peptides which is a characteristic genome feature of these monophyletic avian picornaviruses. The complete genome of a novel picornavirus provisionally named Orivirus A1 (KM203656) from a cloacal sample of a 4-week-old diarrheic chicken (Gallus gallus domesticus) distantly related to members of genus Avihepatovirus was characterized. The study strain contains a type-II-like IRES, a single 2A protein of unknown function unrelated to the 2A proteins of avihepatoviruses and a long 3' untranslated region (UTR) with multiple repeated sequence motifs followed by an AUG-rich region. The repeated sequences of the 3' UTR show significant identity to the "Unit A" sequences of the phylogenetically distant megriviruses. The presence of a novel single 2A and the megrivirus-like "Unit A" motifs suggest multiple recombination events in the evolution of this novel picornavirus.

Original languageEnglish
Pages (from-to)333-338
Number of pages6
JournalInfection, Genetics and Evolution
Volume28
DOIs
Publication statusPublished - Dec 1 2014

Fingerprint

Picornaviridae
3' Untranslated Regions
3' untranslated regions
peptide
Chickens
genome
Genome
chickens
protein
recombination
Proteins
proteins
peptides
Peptides
Ducks
ducks
Genetic Recombination
Avihepatovirus
sampling

Keywords

  • 3' UTR
  • Avian
  • IRES
  • Pathogen
  • Picornavirus
  • Recombination

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Molecular Biology
  • Microbiology
  • Infectious Diseases
  • Microbiology (medical)
  • Medicine(all)

Cite this

Genome characterization of a novel chicken picornavirus distantly related to the members of genus Avihepatovirus with a single 2A protein and a megrivirus-like 3' UTR. / Boros, Ákos; Pankovics, P.; Adonyi, Ádám; Phan, Tung Gia; Delwart, Eric; Reuter, G.

In: Infection, Genetics and Evolution, Vol. 28, 01.12.2014, p. 333-338.

Research output: Contribution to journalArticle

@article{9c45c728300743f0b33fbcc2d272bfa0,
title = "Genome characterization of a novel chicken picornavirus distantly related to the members of genus Avihepatovirus with a single 2A protein and a megrivirus-like 3' UTR",
abstract = "The members of the genus Avihepatovirus and related picornaviruses ({"}Aalivius{"}) of ducks, turkey and chickens possess identical 2A peptide composition including three functionally unrelated 2A peptides which is a characteristic genome feature of these monophyletic avian picornaviruses. The complete genome of a novel picornavirus provisionally named Orivirus A1 (KM203656) from a cloacal sample of a 4-week-old diarrheic chicken (Gallus gallus domesticus) distantly related to members of genus Avihepatovirus was characterized. The study strain contains a type-II-like IRES, a single 2A protein of unknown function unrelated to the 2A proteins of avihepatoviruses and a long 3' untranslated region (UTR) with multiple repeated sequence motifs followed by an AUG-rich region. The repeated sequences of the 3' UTR show significant identity to the {"}Unit A{"} sequences of the phylogenetically distant megriviruses. The presence of a novel single 2A and the megrivirus-like {"}Unit A{"} motifs suggest multiple recombination events in the evolution of this novel picornavirus.",
keywords = "3' UTR, Avian, IRES, Pathogen, Picornavirus, Recombination",
author = "{\'A}kos Boros and P. Pankovics and {\'A}d{\'a}m Adonyi and Phan, {Tung Gia} and Eric Delwart and G. Reuter",
year = "2014",
month = "12",
day = "1",
doi = "10.1016/j.meegid.2014.10.025",
language = "English",
volume = "28",
pages = "333--338",
journal = "Infection, Genetics and Evolution",
issn = "1567-1348",
publisher = "Elsevier",

}

TY - JOUR

T1 - Genome characterization of a novel chicken picornavirus distantly related to the members of genus Avihepatovirus with a single 2A protein and a megrivirus-like 3' UTR

AU - Boros, Ákos

AU - Pankovics, P.

AU - Adonyi, Ádám

AU - Phan, Tung Gia

AU - Delwart, Eric

AU - Reuter, G.

PY - 2014/12/1

Y1 - 2014/12/1

N2 - The members of the genus Avihepatovirus and related picornaviruses ("Aalivius") of ducks, turkey and chickens possess identical 2A peptide composition including three functionally unrelated 2A peptides which is a characteristic genome feature of these monophyletic avian picornaviruses. The complete genome of a novel picornavirus provisionally named Orivirus A1 (KM203656) from a cloacal sample of a 4-week-old diarrheic chicken (Gallus gallus domesticus) distantly related to members of genus Avihepatovirus was characterized. The study strain contains a type-II-like IRES, a single 2A protein of unknown function unrelated to the 2A proteins of avihepatoviruses and a long 3' untranslated region (UTR) with multiple repeated sequence motifs followed by an AUG-rich region. The repeated sequences of the 3' UTR show significant identity to the "Unit A" sequences of the phylogenetically distant megriviruses. The presence of a novel single 2A and the megrivirus-like "Unit A" motifs suggest multiple recombination events in the evolution of this novel picornavirus.

AB - The members of the genus Avihepatovirus and related picornaviruses ("Aalivius") of ducks, turkey and chickens possess identical 2A peptide composition including three functionally unrelated 2A peptides which is a characteristic genome feature of these monophyletic avian picornaviruses. The complete genome of a novel picornavirus provisionally named Orivirus A1 (KM203656) from a cloacal sample of a 4-week-old diarrheic chicken (Gallus gallus domesticus) distantly related to members of genus Avihepatovirus was characterized. The study strain contains a type-II-like IRES, a single 2A protein of unknown function unrelated to the 2A proteins of avihepatoviruses and a long 3' untranslated region (UTR) with multiple repeated sequence motifs followed by an AUG-rich region. The repeated sequences of the 3' UTR show significant identity to the "Unit A" sequences of the phylogenetically distant megriviruses. The presence of a novel single 2A and the megrivirus-like "Unit A" motifs suggest multiple recombination events in the evolution of this novel picornavirus.

KW - 3' UTR

KW - Avian

KW - IRES

KW - Pathogen

KW - Picornavirus

KW - Recombination

UR - http://www.scopus.com/inward/record.url?scp=84918512714&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84918512714&partnerID=8YFLogxK

U2 - 10.1016/j.meegid.2014.10.025

DO - 10.1016/j.meegid.2014.10.025

M3 - Article

C2 - 25445649

AN - SCOPUS:84918512714

VL - 28

SP - 333

EP - 338

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

SN - 1567-1348

ER -