Genetics of sulphate assimilation in Schizosaccharomyces pombe: (A short review)

T. Simonics, Luca Bánszky, Anna Maráz

Research output: Contribution to journalReview article

3 Citations (Scopus)

Abstract

Sulphur plays an important role in yeasts, especially in the biosynthesis of methionine and cysteine. The inorganic sulphur source, sulphate, is taken up by the cells via the sulphate-permease(s). After its transport, it is activated and subsequently reduced to sulphide or serves as a donor for sulphurylation reactions. Selenate anion (SeO42-), which has the same metabolic pathway as sulphate, is toxic for the cells of Schizosaccharomyces pombe. We isolated selenate resistant mutants which cannot utilize sulphate, therefore they need organic sulphur source for growth. One of the selenate resistant mutants was successively transformed with S. pombe genomic libraries and the gene complementing the selenate resistance was identified as that of coding for the ATP-sulphurylase enzyme.

Original languageEnglish
Pages (from-to)279-283
Number of pages5
JournalActa microbiologica et immunologica Hungarica
Volume49
Issue number2-3
DOIs
Publication statusPublished - Dec 1 2002

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Keywords

  • ATP sulphurylase gene
  • S. Pombe
  • Selenate resistant mutants
  • Sulphate utilization

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

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