Genetically timed, activity-sensor and rainbow transsynaptic viral tools

Z. Boldogkői, Kamill Balint, Gautam B. Awatramani, David Balya, Volker Busskamp, Tim James Viney, Pamela S. Lagali, Jens Duebel, Emese Pásti, Dóra Tombácz, J. Tóth, Irma F. Takács, Brigitte Gross Scherf, Botond Roska

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

We developed retrograde, transsynaptic pseudorabies viruses (PRVs) with genetically encoded activity sensors that optically report the activity of connected neurons among spatially intermingled neurons in the brain. Next we engineered PRVs to express two differentially colored fluorescent proteins in a time-shifted manner to define a time period early after infection to investigate neural activity. Finally we used multiple-colored PRVs to differentiate and dissect the complex architecture of brain regions.

Original languageEnglish
Pages (from-to)127-130
Number of pages4
JournalNature Methods
Volume6
Issue number2
DOIs
Publication statusPublished - 2009

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Suid Herpesvirus 1
Viruses
Neurons
Brain
Sensors
Infection
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Boldogkői, Z., Balint, K., Awatramani, G. B., Balya, D., Busskamp, V., Viney, T. J., ... Roska, B. (2009). Genetically timed, activity-sensor and rainbow transsynaptic viral tools. Nature Methods, 6(2), 127-130. https://doi.org/10.1038/nmeth.1292

Genetically timed, activity-sensor and rainbow transsynaptic viral tools. / Boldogkői, Z.; Balint, Kamill; Awatramani, Gautam B.; Balya, David; Busskamp, Volker; Viney, Tim James; Lagali, Pamela S.; Duebel, Jens; Pásti, Emese; Tombácz, Dóra; Tóth, J.; Takács, Irma F.; Scherf, Brigitte Gross; Roska, Botond.

In: Nature Methods, Vol. 6, No. 2, 2009, p. 127-130.

Research output: Contribution to journalArticle

Boldogkői, Z, Balint, K, Awatramani, GB, Balya, D, Busskamp, V, Viney, TJ, Lagali, PS, Duebel, J, Pásti, E, Tombácz, D, Tóth, J, Takács, IF, Scherf, BG & Roska, B 2009, 'Genetically timed, activity-sensor and rainbow transsynaptic viral tools', Nature Methods, vol. 6, no. 2, pp. 127-130. https://doi.org/10.1038/nmeth.1292
Boldogkői, Z. ; Balint, Kamill ; Awatramani, Gautam B. ; Balya, David ; Busskamp, Volker ; Viney, Tim James ; Lagali, Pamela S. ; Duebel, Jens ; Pásti, Emese ; Tombácz, Dóra ; Tóth, J. ; Takács, Irma F. ; Scherf, Brigitte Gross ; Roska, Botond. / Genetically timed, activity-sensor and rainbow transsynaptic viral tools. In: Nature Methods. 2009 ; Vol. 6, No. 2. pp. 127-130.
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