Genetic variability of Candida albicans isolates in a university hospital in Hungary

Zoltán Farkas, S. Kocsubé, Mónika Tóth, C. Vágvölgyi, J. Kucsera, J. Varga, I. Pfeiffer

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The occurrence and genetic variability of Candida albicans isolates in a Hungarian hospital were examined. Among the 103 Candida isolates, 44 (42.7%) proved to be C. albicans species. Comparing with a previous study carried out in 2002, the percentage of infections caused by C. albicans decreased in Hungary in this period with an increasing incidence of non-albicans species, in accordance with the world-wide trend. The genetic variability of the isolates was examined using mitochondrial DNA (mtDNA), restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) analysis and electrophoretic karyotyping. The examined C. albicans isolates could be clustered into four groups based on their mtDNA profiles. The electrophoretic karyotypes of the isolates were mostly identical to that of the reference strain 1006, with the exception of mtDNA type II isolates. RAPD analysis could be used to cluster the isolates into different groups, but this clustering was not in complete agreement with their assignment to mtDNA types. Population genetic analyses of the data indicated low amounts of recombination among these C. albicans strains. None of the isolates exhibited decreased susceptibilities to 5-fluorocytosine.

Original languageEnglish
Pages (from-to)318-325
Number of pages8
JournalMycoses
Volume52
Issue number4
DOIs
Publication statusPublished - Jul 2009

Fingerprint

Hungary
Candida albicans
Mitochondrial DNA
Flucytosine
Karyotyping
DNA
Population Genetics
Karyotype
Candida
Restriction Fragment Length Polymorphisms
Genetic Recombination
Cluster Analysis
Incidence
Infection

Keywords

  • Candida albicans
  • Genetic variability

ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases

Cite this

Genetic variability of Candida albicans isolates in a university hospital in Hungary. / Farkas, Zoltán; Kocsubé, S.; Tóth, Mónika; Vágvölgyi, C.; Kucsera, J.; Varga, J.; Pfeiffer, I.

In: Mycoses, Vol. 52, No. 4, 07.2009, p. 318-325.

Research output: Contribution to journalArticle

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