Genetic variability among serotype G6 human rotaviruses: Identification of a novel lineage isolated in Hungary

K. Bányai, Jon R. Gentsch, Dixie D. Griffin, Jennifer L. Holmes, Roger I. Glass, György Szücs

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Rotavirus serotype G6 has been demonstrated to be a rare cause of gastroenteritis in man. To date, only a few well characterized strains have been described from Italy, Australia, and the United States. Nucleotide sequencing of G6 VP7 genes shows that these strains belong to two distinct G6 lineages, one for strains of serotype P11[14],G6 (PA169-like strains) and one for strains of serotype P3[9],G6 (PA151-like strains). In this study, we sequenced the VP7 genes and VP8* gene fragments of human rotavirus G6 strains detected in Hungary. Phylogenetic analysis demonstrated that the VP7 genes of Hungarian G6 strains fell into three lineages, represented by a single PA169-like strain, three PA151-like strains, and two novel G6 strains, respectively. The amino acid sequence identity of VP7 was 97.2-100% within each lineage and 92-93.9% between any two lineages. The sequence analysis of VP8* revealed that the single PA169-like Hungarian G6 strain belonged to genotype P[14] and was phylogenetically closely related to P11[14],G6 strains characterized previously. In contrast, the VP8* of PA151-like Hungarian G6 strains clustered in accordance with their VP7 genes representing genetically distinguishable variants of genotype P[9]. This finding raises the possibility that Hungarian genotype P[9],G6 strains might have been generated through independent reassortment events. Serotype G6-specific primers for each human G6 lineage were also developed. The use of these primers in reverse-transcription polymerase chain reaction genotyping may help determine the epidemiological role of G6 strains in humans.

Original languageEnglish
Pages (from-to)124-134
Number of pages11
JournalJournal of Medical Virology
Volume71
Issue number1
DOIs
Publication statusPublished - Sep 1 2003

Fingerprint

Forensic Anthropology
Hungary
Rotavirus
Genes
Genotype
Gastroenteritis
Italy
Reverse Transcription
Sequence Analysis
Amino Acid Sequence
Nucleotides
Serogroup
Polymerase Chain Reaction

Keywords

  • Genotyping
  • RT-PCR
  • Sequence analysis
  • VP4
  • VP7

ASJC Scopus subject areas

  • Virology

Cite this

Genetic variability among serotype G6 human rotaviruses : Identification of a novel lineage isolated in Hungary. / Bányai, K.; Gentsch, Jon R.; Griffin, Dixie D.; Holmes, Jennifer L.; Glass, Roger I.; Szücs, György.

In: Journal of Medical Virology, Vol. 71, No. 1, 01.09.2003, p. 124-134.

Research output: Contribution to journalArticle

Bányai, K. ; Gentsch, Jon R. ; Griffin, Dixie D. ; Holmes, Jennifer L. ; Glass, Roger I. ; Szücs, György. / Genetic variability among serotype G6 human rotaviruses : Identification of a novel lineage isolated in Hungary. In: Journal of Medical Virology. 2003 ; Vol. 71, No. 1. pp. 124-134.
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