Genetic technologies for the identification of plant genes controlling environmental stress responses

Csaba Papdi, Mary Prathiba Joseph, Imma Pérez Salamó, Sabina Vidal, L. Szabados

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Abiotic conditions such as light, temperature, water availability and soil parameters determine plant growth and development. The adaptation of plants to extreme environments or to sudden changes in their growth conditions is controlled by a well balanced, genetically determined signalling system, which is still far from being understood. The identification and characterisation of plant genes which control responses to environmental stresses is an essential step to elucidate the complex regulatory network, which determines stress tolerance. Here, we review the genetic approaches, which have been used with success to identify plant genes which control responses to different abiotic stress factors. We describe strategies and concepts for forward and reverse genetic screens, conventional and insertion mutagenesis, TILLING, gene tagging, promoter trapping, activation mutagenesis and cDNA library transfer. The utility of the various genetic approaches in plant stress research we review is illustrated by several published examples.

Original languageEnglish
Pages (from-to)696-720
Number of pages25
JournalFunctional Plant Biology
Volume36
Issue number8
DOIs
Publication statusPublished - 2009

Fingerprint

stress response
mutagenesis
genes
plant adaptation
plant stress
cDNA libraries
stress tolerance
abiotic stress
trapping
plant development
growth and development
water temperature
promoter regions
plant growth
plant identification
soil

Keywords

  • Abiotic stress
  • Activation
  • Arabidopsis
  • Cold
  • Drought
  • Forward genetics
  • Gene
  • Gene tagging
  • Genetic screen
  • Heat stress mutagenesis
  • Osmotic stress
  • Oxidative stress
  • Reverse genetics
  • Salinity
  • Variability.

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Genetic technologies for the identification of plant genes controlling environmental stress responses. / Papdi, Csaba; Joseph, Mary Prathiba; Salamó, Imma Pérez; Vidal, Sabina; Szabados, L.

In: Functional Plant Biology, Vol. 36, No. 8, 2009, p. 696-720.

Research output: Contribution to journalArticle

Papdi, Csaba ; Joseph, Mary Prathiba ; Salamó, Imma Pérez ; Vidal, Sabina ; Szabados, L. / Genetic technologies for the identification of plant genes controlling environmental stress responses. In: Functional Plant Biology. 2009 ; Vol. 36, No. 8. pp. 696-720.
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