Genetic Polymorphism of Milk Proteins in Hungarian Spotted and Hungarian Grey Cattle

A Possible New Genetic Variant of β-Lactoglobulin

M. Baranyi, Z. Bösze, J. Buchberger, I. Krause

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Genetic variants of αs1-, β-, and κ-caseins and of β-lactoglobulin were studied with PAGE and isoelectric focusing in milk samples of 101 Hungarian Spotted and 120 Hungarian Grey cows. Gene frequencies and genotypic frequencies were estimated. Significant differences in gene frequencies between the two breeds were observed at the κ-casein and β-lactoglobulin locus. The variants β-casein C, κ-casein C, and β-lactoglobulin D were not found in Hungarian Grey cows. On immobilized pH gradient, isoelectric focusing gels containing carrier ampholytes, a new unidentified protein band appeared near β-lactoglobulin B in about 10% of the milk samples of Hungarian Grey cattle.

Original languageEnglish
Pages (from-to)630-636
Number of pages7
JournalJournal of Dairy Science
Volume76
Issue number2
DOIs
Publication statusPublished - Jan 1 1993

Fingerprint

lactoglobulins
Lactoglobulins
Milk Proteins
milk proteins
Genetic Polymorphisms
Caseins
casein
genetic polymorphism
cattle
isoelectric focusing
Isoelectric Focusing
Gene Frequency
gene frequency
Milk
Ampholyte Mixtures
cows
milk
Proton-Motive Force
polyacrylamide gel electrophoresis
Gels

Keywords

  • CA-IEF
  • CA-IPG-IEF
  • casein (used with α-, α-, β-, and κ-)
  • CN
  • genetic polymorphism
  • HG
  • HS
  • Hungarian cattle
  • Hungarian Grey
  • Hungarian Spotted
  • isoelectric focusing in immobilized pH gradients in the presence of carrier ampholytes
  • isoelectric focusing in the presence of carrier ampholytes
  • lactoglobulin (used with β-)
  • LG
  • milk protein

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Genetic Polymorphism of Milk Proteins in Hungarian Spotted and Hungarian Grey Cattle : A Possible New Genetic Variant of β-Lactoglobulin. / Baranyi, M.; Bösze, Z.; Buchberger, J.; Krause, I.

In: Journal of Dairy Science, Vol. 76, No. 2, 01.01.1993, p. 630-636.

Research output: Contribution to journalArticle

@article{24d70dcb48a64f2eaf503fe5b7f32d81,
title = "Genetic Polymorphism of Milk Proteins in Hungarian Spotted and Hungarian Grey Cattle: A Possible New Genetic Variant of β-Lactoglobulin",
abstract = "Genetic variants of αs1-, β-, and κ-caseins and of β-lactoglobulin were studied with PAGE and isoelectric focusing in milk samples of 101 Hungarian Spotted and 120 Hungarian Grey cows. Gene frequencies and genotypic frequencies were estimated. Significant differences in gene frequencies between the two breeds were observed at the κ-casein and β-lactoglobulin locus. The variants β-casein C, κ-casein C, and β-lactoglobulin D were not found in Hungarian Grey cows. On immobilized pH gradient, isoelectric focusing gels containing carrier ampholytes, a new unidentified protein band appeared near β-lactoglobulin B in about 10{\%} of the milk samples of Hungarian Grey cattle.",
keywords = "CA-IEF, CA-IPG-IEF, casein (used with α-, α-, β-, and κ-), CN, genetic polymorphism, HG, HS, Hungarian cattle, Hungarian Grey, Hungarian Spotted, isoelectric focusing in immobilized pH gradients in the presence of carrier ampholytes, isoelectric focusing in the presence of carrier ampholytes, lactoglobulin (used with β-), LG, milk protein",
author = "M. Baranyi and Z. B{\"o}sze and J. Buchberger and I. Krause",
year = "1993",
month = "1",
day = "1",
doi = "10.3168/jds.S0022-0302(93)77384-1",
language = "English",
volume = "76",
pages = "630--636",
journal = "Journal of Dairy Science",
issn = "0022-0302",
publisher = "Elsevier Limited",
number = "2",

}

TY - JOUR

T1 - Genetic Polymorphism of Milk Proteins in Hungarian Spotted and Hungarian Grey Cattle

T2 - A Possible New Genetic Variant of β-Lactoglobulin

AU - Baranyi, M.

AU - Bösze, Z.

AU - Buchberger, J.

AU - Krause, I.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Genetic variants of αs1-, β-, and κ-caseins and of β-lactoglobulin were studied with PAGE and isoelectric focusing in milk samples of 101 Hungarian Spotted and 120 Hungarian Grey cows. Gene frequencies and genotypic frequencies were estimated. Significant differences in gene frequencies between the two breeds were observed at the κ-casein and β-lactoglobulin locus. The variants β-casein C, κ-casein C, and β-lactoglobulin D were not found in Hungarian Grey cows. On immobilized pH gradient, isoelectric focusing gels containing carrier ampholytes, a new unidentified protein band appeared near β-lactoglobulin B in about 10% of the milk samples of Hungarian Grey cattle.

AB - Genetic variants of αs1-, β-, and κ-caseins and of β-lactoglobulin were studied with PAGE and isoelectric focusing in milk samples of 101 Hungarian Spotted and 120 Hungarian Grey cows. Gene frequencies and genotypic frequencies were estimated. Significant differences in gene frequencies between the two breeds were observed at the κ-casein and β-lactoglobulin locus. The variants β-casein C, κ-casein C, and β-lactoglobulin D were not found in Hungarian Grey cows. On immobilized pH gradient, isoelectric focusing gels containing carrier ampholytes, a new unidentified protein band appeared near β-lactoglobulin B in about 10% of the milk samples of Hungarian Grey cattle.

KW - CA-IEF

KW - CA-IPG-IEF

KW - casein (used with α-, α-, β-, and κ-)

KW - CN

KW - genetic polymorphism

KW - HG

KW - HS

KW - Hungarian cattle

KW - Hungarian Grey

KW - Hungarian Spotted

KW - isoelectric focusing in immobilized pH gradients in the presence of carrier ampholytes

KW - isoelectric focusing in the presence of carrier ampholytes

KW - lactoglobulin (used with β-)

KW - LG

KW - milk protein

UR - http://www.scopus.com/inward/record.url?scp=0000824444&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000824444&partnerID=8YFLogxK

U2 - 10.3168/jds.S0022-0302(93)77384-1

DO - 10.3168/jds.S0022-0302(93)77384-1

M3 - Article

VL - 76

SP - 630

EP - 636

JO - Journal of Dairy Science

JF - Journal of Dairy Science

SN - 0022-0302

IS - 2

ER -