Allélpolimorfizmusok vizsgálata colorectalis és fej-nyak táji daganatos betegekben

Translated title of the contribution: Genetic polymorphism in patients with colorectal and with head and neck cancer

András Csejtei, Antal Tibold, I. Ember, István Kiss

Research output: Contribution to journalArticle

Abstract

Colorectal cancer is the second most frequent cause of death among malignant diseases. The mortality of head and neck cancer in Hungary increased by 265 percent in the last thirty years. Both malignancies belong to the most current public health problems in Hungary. The influence of two allelic polymorphisms of GSTM1 and GSTT1, and that of p53 gene codon 72 on colon cancer was investigated. In case of head and neck cancer the effects of the Arg194Trp and Arg399Gln polymorphisms of XRCC1 gene were analyzed. Intraoperative removed tissue samples were processed and cancer free human samples were used as matched controls. The formalin fixed samples were deparaffinized and digested with proteinase K. Genotyping was performed by PCR amplification, and in case of head and neck cancer a PCR-RFLP method was applied. No significant difference was found between tumor patients and controls in the investigated polymorphisms. A significant difference in survival was found between the GSTM1 and p53 gene variants in Dukes'B stage colorectal patients. The survival difference among the XRCC1 194 alleles by head and neck patients in clinical stage III proved to be also significant. The complex analysis of this type of genetic variants may be the future way of the personal risk assessment and the real chance for personal therapy.

Original languageHungarian
Pages (from-to)1545-1549
Number of pages5
JournalOrvosi Hetilap
Volume150
Issue number33
DOIs
Publication statusPublished - Aug 1 2009

Fingerprint

Genetic Polymorphisms
Head and Neck Neoplasms
Hungary
p53 Genes
Endopeptidase K
Neoplasms
Polymerase Chain Reaction
Survival
Codon
Restriction Fragment Length Polymorphisms
Colonic Neoplasms
Formaldehyde
Cause of Death
Colorectal Neoplasms
Neck
Public Health
Alleles
Head
Mortality
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Allélpolimorfizmusok vizsgálata colorectalis és fej-nyak táji daganatos betegekben. / Csejtei, András; Tibold, Antal; Ember, I.; Kiss, István.

In: Orvosi Hetilap, Vol. 150, No. 33, 01.08.2009, p. 1545-1549.

Research output: Contribution to journalArticle

Csejtei, András ; Tibold, Antal ; Ember, I. ; Kiss, István. / Allélpolimorfizmusok vizsgálata colorectalis és fej-nyak táji daganatos betegekben. In: Orvosi Hetilap. 2009 ; Vol. 150, No. 33. pp. 1545-1549.
@article{727ab052cb4e4a01ae908dc60174d9d0,
title = "All{\'e}lpolimorfizmusok vizsg{\'a}lata colorectalis {\'e}s fej-nyak t{\'a}ji daganatos betegekben",
abstract = "Colorectal cancer is the second most frequent cause of death among malignant diseases. The mortality of head and neck cancer in Hungary increased by 265 percent in the last thirty years. Both malignancies belong to the most current public health problems in Hungary. The influence of two allelic polymorphisms of GSTM1 and GSTT1, and that of p53 gene codon 72 on colon cancer was investigated. In case of head and neck cancer the effects of the Arg194Trp and Arg399Gln polymorphisms of XRCC1 gene were analyzed. Intraoperative removed tissue samples were processed and cancer free human samples were used as matched controls. The formalin fixed samples were deparaffinized and digested with proteinase K. Genotyping was performed by PCR amplification, and in case of head and neck cancer a PCR-RFLP method was applied. No significant difference was found between tumor patients and controls in the investigated polymorphisms. A significant difference in survival was found between the GSTM1 and p53 gene variants in Dukes'B stage colorectal patients. The survival difference among the XRCC1 194 alleles by head and neck patients in clinical stage III proved to be also significant. The complex analysis of this type of genetic variants may be the future way of the personal risk assessment and the real chance for personal therapy.",
keywords = "Colorectal cancer, GSTM1, GSTT1, Head and neck cancer, P53, Polymorphisms, Survival, XRCC1 194, XRCC1 399",
author = "Andr{\'a}s Csejtei and Antal Tibold and I. Ember and Istv{\'a}n Kiss",
year = "2009",
month = "8",
day = "1",
doi = "10.1556/OH.2009.28634",
language = "Hungarian",
volume = "150",
pages = "1545--1549",
journal = "Orvosi Hetilap",
issn = "0030-6002",
publisher = "Akademiai Kiado",
number = "33",

}

TY - JOUR

T1 - Allélpolimorfizmusok vizsgálata colorectalis és fej-nyak táji daganatos betegekben

AU - Csejtei, András

AU - Tibold, Antal

AU - Ember, I.

AU - Kiss, István

PY - 2009/8/1

Y1 - 2009/8/1

N2 - Colorectal cancer is the second most frequent cause of death among malignant diseases. The mortality of head and neck cancer in Hungary increased by 265 percent in the last thirty years. Both malignancies belong to the most current public health problems in Hungary. The influence of two allelic polymorphisms of GSTM1 and GSTT1, and that of p53 gene codon 72 on colon cancer was investigated. In case of head and neck cancer the effects of the Arg194Trp and Arg399Gln polymorphisms of XRCC1 gene were analyzed. Intraoperative removed tissue samples were processed and cancer free human samples were used as matched controls. The formalin fixed samples were deparaffinized and digested with proteinase K. Genotyping was performed by PCR amplification, and in case of head and neck cancer a PCR-RFLP method was applied. No significant difference was found between tumor patients and controls in the investigated polymorphisms. A significant difference in survival was found between the GSTM1 and p53 gene variants in Dukes'B stage colorectal patients. The survival difference among the XRCC1 194 alleles by head and neck patients in clinical stage III proved to be also significant. The complex analysis of this type of genetic variants may be the future way of the personal risk assessment and the real chance for personal therapy.

AB - Colorectal cancer is the second most frequent cause of death among malignant diseases. The mortality of head and neck cancer in Hungary increased by 265 percent in the last thirty years. Both malignancies belong to the most current public health problems in Hungary. The influence of two allelic polymorphisms of GSTM1 and GSTT1, and that of p53 gene codon 72 on colon cancer was investigated. In case of head and neck cancer the effects of the Arg194Trp and Arg399Gln polymorphisms of XRCC1 gene were analyzed. Intraoperative removed tissue samples were processed and cancer free human samples were used as matched controls. The formalin fixed samples were deparaffinized and digested with proteinase K. Genotyping was performed by PCR amplification, and in case of head and neck cancer a PCR-RFLP method was applied. No significant difference was found between tumor patients and controls in the investigated polymorphisms. A significant difference in survival was found between the GSTM1 and p53 gene variants in Dukes'B stage colorectal patients. The survival difference among the XRCC1 194 alleles by head and neck patients in clinical stage III proved to be also significant. The complex analysis of this type of genetic variants may be the future way of the personal risk assessment and the real chance for personal therapy.

KW - Colorectal cancer

KW - GSTM1

KW - GSTT1

KW - Head and neck cancer

KW - P53

KW - Polymorphisms

KW - Survival

KW - XRCC1 194

KW - XRCC1 399

UR - http://www.scopus.com/inward/record.url?scp=70349248038&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349248038&partnerID=8YFLogxK

U2 - 10.1556/OH.2009.28634

DO - 10.1556/OH.2009.28634

M3 - Article

C2 - 19643719

AN - SCOPUS:70349248038

VL - 150

SP - 1545

EP - 1549

JO - Orvosi Hetilap

JF - Orvosi Hetilap

SN - 0030-6002

IS - 33

ER -