Genetic link between p53 and genes required for formation of the zonula adherens junction

Masamitsu Yamaguchi, Fumiko Hirose, Yoshihiro H. Inoue, Katsuhito Ohno, Hideki Yoshida, Yuko Hayashi, P. Deák, Akio Matsukage

Research output: Contribution to journalArticle

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Abstract

Ectopic expression of human p53 in Drosophila eye imaginal disc cells induces apoptosis and results in a rough eye phenotype in the adult flies. We have screened Drosophila stocks to identify mutations that enhance or suppress the p53-induced rough eye phenotype. One of the dominant enhancers of the p53-induced rough eye phenotype corresponds to a loss-of-function mutation of the crumbs gene, which is essential for the biogenesis of the zonula adherens junction and the establishment of apical polarity in epithelial cells. Enhancement of p53-induced apoptosis in the eye imaginal discs by a half-reduction of the crumbs gene dose was confirmed by a TUNEL method. Furthermore, mutations of genes for Shotgun (Drosophila E-cadherin) and Armadillo (Drosophila β-catenin), the two main components of the adherens junction, also strongly enhanced the p53-induced rough eye phenotype. These results suggest that human p53 senses subtle abnormality at the adherens junction or in signals derived from the junction, and consequently induces apoptosis to remove abnormal cells from tissue. Thus p53 likely plays a role as a guardian of the tissue not only by sensing the damaged DNA, but also by sensing signals from the adherens junction.

Original languageEnglish
Pages (from-to)436-441
Number of pages6
JournalCancer Science
Volume95
Issue number5
DOIs
Publication statusPublished - May 2004

Fingerprint

Adherens Junctions
p53 Genes
Drosophila
Imaginal Discs
Phenotype
Apoptosis
Mutation
Armadillos
Genes
Catenins
In Situ Nick-End Labeling
Firearms
Cadherins
Diptera
Epithelial Cells
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Yamaguchi, M., Hirose, F., Inoue, Y. H., Ohno, K., Yoshida, H., Hayashi, Y., ... Matsukage, A. (2004). Genetic link between p53 and genes required for formation of the zonula adherens junction. Cancer Science, 95(5), 436-441. https://doi.org/10.1111/j.1349-7006.2004.tb03228.x

Genetic link between p53 and genes required for formation of the zonula adherens junction. / Yamaguchi, Masamitsu; Hirose, Fumiko; Inoue, Yoshihiro H.; Ohno, Katsuhito; Yoshida, Hideki; Hayashi, Yuko; Deák, P.; Matsukage, Akio.

In: Cancer Science, Vol. 95, No. 5, 05.2004, p. 436-441.

Research output: Contribution to journalArticle

Yamaguchi, M, Hirose, F, Inoue, YH, Ohno, K, Yoshida, H, Hayashi, Y, Deák, P & Matsukage, A 2004, 'Genetic link between p53 and genes required for formation of the zonula adherens junction', Cancer Science, vol. 95, no. 5, pp. 436-441. https://doi.org/10.1111/j.1349-7006.2004.tb03228.x
Yamaguchi, Masamitsu ; Hirose, Fumiko ; Inoue, Yoshihiro H. ; Ohno, Katsuhito ; Yoshida, Hideki ; Hayashi, Yuko ; Deák, P. ; Matsukage, Akio. / Genetic link between p53 and genes required for formation of the zonula adherens junction. In: Cancer Science. 2004 ; Vol. 95, No. 5. pp. 436-441.
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