Genetic interrelationships and genome organization of double-stranded RNA elements of Fusarium poae

Peter Compel, István Papp, Márton Bibó, Csaba Fekete, László Hornok

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The similar-sized double-stranded RNA (dsRNA) elements present in vegetatively compatible strains of Fusarium poae were always genetically related, while vegetatively incompatible strains of the fungus contained either homologous or non-homologous dsRNAs of the same size. Electron microscopic observations revealed the co-existence of encapsidated and naked dsRNA elements in the same host. A mycovirus, named FUPO-1 was purified from strain A-11 and was found to contain two kinds of dsRNA segments, dsRNA 1 and dsRNA 2. The dsRNA genome of these segments was converted to cDNA clones by reverse transcription and the clones were subjected to sequence analysis. The single long open reading frame deduced from the sequence of dsRNA 1 showed similarities to the putative coat protein genes known from other mycoviruses, while conserved motifs of an RNA-dependent RNA polymerase were identified in the predicted amino acid sequence of dsRNA 2. The genome organization and certain sequence motifs of FUPO-1 show similarities to that of the Atkinsonella hypoxylon 2H virus and the Fuso V mycovirus, members of the Partitviridae family.

Original languageEnglish
Pages (from-to)49-56
Number of pages8
JournalVirus Genes
Volume18
Issue number1
DOIs
Publication statusPublished - Mar 30 1999

    Fingerprint

Keywords

  • Fusarium
  • Genome organization
  • Mycovirus
  • Nucleotide sequence

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

Cite this