Genetic heterogeneity and recombination in type-3 human astroviruses

Maria Cristina Medici, Fabio Tummolo, Vito Martella, K. Bányai, Elisabetta Bonerba, Carlo Chezzi, Maria Cristina Arcangeletti, Flora De Conto, Adriana Calderaro

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Human astroviruses (HAstVs) are important enteric pathogens and can be classified genetically and antigenically into eight types. During molecular surveillance for HAstVs in Italy, sequence analysis of the diagnostic region C (about 400 nucleotide in length), located on the capsid (ORF2) gene, identified a novel type-3 strain. Upon sequencing of the full-length ORF2, the type-3 HAstV strain was characterized as a novel ORF2 genetic lineage, designated as 3c. By converse, in the ORF1b the virus was more similar to type-1 HAstVs, rather than to type-3 strains, suggesting a recombination nature, with the crossover site being mapped to the ORF1b/ORF2 junction region. Region C sequences of similar type-3 HAstV identified from European and extra-European countries were retrieved in the databases, suggesting the global distribution of this novel type-3 lineage.

Original languageEnglish
Pages (from-to)156-160
Number of pages5
JournalInfection, Genetics and Evolution
Volume32
DOIs
Publication statusPublished - Jun 1 2015

Fingerprint

Human astrovirus
Mamastrovirus
genetic recombination
Genetic Heterogeneity
Genetic Recombination
recombination
Capsid
Italy
Sequence Analysis
virus
Nucleotides
pathogen
Databases
capsid
Viruses
gene
sequence analysis
Genes
nucleotides
viruses

Keywords

  • Astrovirus
  • Genotyping
  • Italy
  • Recombination
  • Viral gastroenteritis

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Molecular Biology
  • Microbiology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Medici, M. C., Tummolo, F., Martella, V., Bányai, K., Bonerba, E., Chezzi, C., ... Calderaro, A. (2015). Genetic heterogeneity and recombination in type-3 human astroviruses. Infection, Genetics and Evolution, 32, 156-160. https://doi.org/10.1016/j.meegid.2015.03.011

Genetic heterogeneity and recombination in type-3 human astroviruses. / Medici, Maria Cristina; Tummolo, Fabio; Martella, Vito; Bányai, K.; Bonerba, Elisabetta; Chezzi, Carlo; Arcangeletti, Maria Cristina; De Conto, Flora; Calderaro, Adriana.

In: Infection, Genetics and Evolution, Vol. 32, 01.06.2015, p. 156-160.

Research output: Contribution to journalArticle

Medici, MC, Tummolo, F, Martella, V, Bányai, K, Bonerba, E, Chezzi, C, Arcangeletti, MC, De Conto, F & Calderaro, A 2015, 'Genetic heterogeneity and recombination in type-3 human astroviruses', Infection, Genetics and Evolution, vol. 32, pp. 156-160. https://doi.org/10.1016/j.meegid.2015.03.011
Medici, Maria Cristina ; Tummolo, Fabio ; Martella, Vito ; Bányai, K. ; Bonerba, Elisabetta ; Chezzi, Carlo ; Arcangeletti, Maria Cristina ; De Conto, Flora ; Calderaro, Adriana. / Genetic heterogeneity and recombination in type-3 human astroviruses. In: Infection, Genetics and Evolution. 2015 ; Vol. 32. pp. 156-160.
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