Genetic enhancement of the ability to tolerate photoinhibition by introduction of unsaturated bonds into membrane glycerolipids

Z. Gombos, Eira Kanervo, Nelly Tsvetkova, Toshio Sakamoto, Eva Mari Aro, Norio Murata

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Strong light leads to damage to photosynthetic machinery, particularly at low temperatures, and the main site of the damage is the D1 protein of the photosystem II (PSII) complex. Here we describe that transformation of Synechococcus sp. PCC 7942 with the desA gene for a Δ12 desaturase increased unsaturation of membrane lipids and enhanced tolerance to strong light. To our knowledge, this is the first report of the successful genetic enhancement of tolerance to strong light. Analysis of the light-induced inactivation and of the subsequent recovery of the activity of the PSII complex revealed that the recovery process was markedly accelerated by the genetic transformation. Labeling experiments with [35S]L-methionine also revealed that the synthesis of the D1 protein de novo at low temperature, which was a prerequisite for the restoration of the PSII complex, was much faster in the transformed cells than in the wild-type cells. These findings demonstrate that the ability of membrane lipids to desaturate fatty acids is important for the photosynthetic organisms to tolerate strong light, by accelerating the synthesis of the D1 protein de novo.

Original languageEnglish
Pages (from-to)551-559
Number of pages9
JournalPlant Physiology
Volume115
Issue number2
Publication statusPublished - 1997

Fingerprint

Genetic Enhancement
photoinhibition
D1 protein
Photosystem II Protein Complex
Light
Membranes
photosystem II
Membrane Lipids
Synechococcus sp. PCC 7942
Synechococcus
Genetic Transformation
Temperature
synthesis
autotrophs
genetic transformation
lipids
Methionine
methionine
inactivation
temperature

ASJC Scopus subject areas

  • Plant Science

Cite this

Genetic enhancement of the ability to tolerate photoinhibition by introduction of unsaturated bonds into membrane glycerolipids. / Gombos, Z.; Kanervo, Eira; Tsvetkova, Nelly; Sakamoto, Toshio; Aro, Eva Mari; Murata, Norio.

In: Plant Physiology, Vol. 115, No. 2, 1997, p. 551-559.

Research output: Contribution to journalArticle

Gombos, Z, Kanervo, E, Tsvetkova, N, Sakamoto, T, Aro, EM & Murata, N 1997, 'Genetic enhancement of the ability to tolerate photoinhibition by introduction of unsaturated bonds into membrane glycerolipids', Plant Physiology, vol. 115, no. 2, pp. 551-559.
Gombos, Z. ; Kanervo, Eira ; Tsvetkova, Nelly ; Sakamoto, Toshio ; Aro, Eva Mari ; Murata, Norio. / Genetic enhancement of the ability to tolerate photoinhibition by introduction of unsaturated bonds into membrane glycerolipids. In: Plant Physiology. 1997 ; Vol. 115, No. 2. pp. 551-559.
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