Genetic diversity of Bacillus thuringiensis serovars revealed by RFLP using random DNA probes

Claudia Wrtz Jrgensen, Nicklas Raun Jacobsen, T. Emri, Susanne Havn Eriksen, I. Pócsi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

EcoRI and HindIII restriction fragment length polymorphism (RFLP) profiles using 2 random DNA probes, named 104 and 106, were generated for 85 B. thuringiensis strains. These include 80 serovars, 4 intra-serovar strains: kurstaki HD-1, dendrolimus, tenebrionis and sandiego, and a non-serotypeable strain B. thuringiensis var. wuhanensis. A total of 47 EcoRI and 65 HindIII restriction patterns were generated when hybridization results from both probes were combined. Seventy-seven B. thuringiensis strains showed distinctive hybridization profiles. The dendrogram resulting from the numerical analysis of the distance matrix revealed fourteen distinct phylogenetic groups at the 96% banding patterns similarity. The intra-serovar strains showed higher similarity with their respective type serovars. However, different serovars from a common H-serotype did not always cluster in the same phylogenetic group. Alternatively, several mosquitocidal serovars clustered in a single phylogenetic group. The correlation between serotyping and banding pattern similarity is discussed.

Original languageEnglish
Pages (from-to)85-95
Number of pages11
JournalJournal of Basic Microbiology
Volume41
Issue number2
DOIs
Publication statusPublished - 2001

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Bacillus thuringiensis
DNA Probes
Restriction Fragment Length Polymorphisms
Serotyping
Serogroup

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Genetics

Cite this

Genetic diversity of Bacillus thuringiensis serovars revealed by RFLP using random DNA probes. / Jrgensen, Claudia Wrtz; Jacobsen, Nicklas Raun; Emri, T.; Eriksen, Susanne Havn; Pócsi, I.

In: Journal of Basic Microbiology, Vol. 41, No. 2, 2001, p. 85-95.

Research output: Contribution to journalArticle

Jrgensen, Claudia Wrtz ; Jacobsen, Nicklas Raun ; Emri, T. ; Eriksen, Susanne Havn ; Pócsi, I. / Genetic diversity of Bacillus thuringiensis serovars revealed by RFLP using random DNA probes. In: Journal of Basic Microbiology. 2001 ; Vol. 41, No. 2. pp. 85-95.
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