Spleens from normal young mice contain lymphocytes that can kill certain in vitro grown Moloney lymphoma lines in a51Cr-release cytotoxicity test. A lymphoid cell without detectable T- or B-cell markers was previously shown to be responsible. Killing activity shows a marked dependence on the genotype of the donor mouse. When tested against a YAC line of strain A origin maintained in vitro spleens of A, A.CA, and A.SW mice had low activity, whereas CBA, C3H, C57L, and C57Bl spleens were highly active. In semisyngeneic F1 crosses with strain A as one parent, reactivity resembled the opposite parental strain. Thus, (A×CBA)F1, (A×C3H)F1, (A×C57L)F1, and (A×C57Bl)F1 were reactive, whereas A×A.CA showed no significant activity. Analysis of the reactivity in (A×C57Bl)F1×A backcross mice suggests that multiple genes are involved. Preliminary linkage analysis suggests at least one H-2 linked factor. Another gene appears to be linked to the B (black) locus.
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