Genetic characterization of the region between 86F1,2 and 87B15 on chromosome 3 of Drosophila melanogaster.

J. Gausz, H. Gyurkovics, G. Bencze, A. A. Awad, J. J. Holden, D. Ish-Horowicz

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Abstract

The region between 86F1,2 and 87B15 on chromosome 3 of Drosophila melanogaster, which contains about 27 polytene chromosome bands including the 87A7 heat-shock locus, has been screened for EMS-induced visible and lethal mutations. We have recovered 268 lethal mutations that fall into 25 complementation groups. Cytogenetic localization of the complementation groups by deficiency mapping is consistent with the notion that each band encodes a single genetic function. We have also screened for mutations at the 87A7 heat shock locus, using a chromosome that has only one copy of the gene encoding the 70,000 dalton heat-shock protein (hsp70). No lethal or visible mutations at 87A7 were identified from 10,719 mutagenized chromosomes, and no female-sterile mutations at 87A7 were recovered from the 1,520 chromosomes whose progeny were tested for female fertility. We found no evidence that a functional hsp70 gene is required for development under laboratory conditions.

Original languageEnglish
Pages (from-to)775-789
Number of pages15
JournalGenetics
Volume98
Issue number4
Publication statusPublished - Aug 1981

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Chromosomes, Human, Pair 3
Drosophila melanogaster
Mutation
Shock
Hot Temperature
Chromosomes
Polytene Chromosomes
Chromosomes, Human, Pair 1
Heat-Shock Proteins
Cytogenetics
Genes
Fertility

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Gausz, J., Gyurkovics, H., Bencze, G., Awad, A. A., Holden, J. J., & Ish-Horowicz, D. (1981). Genetic characterization of the region between 86F1,2 and 87B15 on chromosome 3 of Drosophila melanogaster. Genetics, 98(4), 775-789.

Genetic characterization of the region between 86F1,2 and 87B15 on chromosome 3 of Drosophila melanogaster. / Gausz, J.; Gyurkovics, H.; Bencze, G.; Awad, A. A.; Holden, J. J.; Ish-Horowicz, D.

In: Genetics, Vol. 98, No. 4, 08.1981, p. 775-789.

Research output: Contribution to journalArticle

Gausz, J, Gyurkovics, H, Bencze, G, Awad, AA, Holden, JJ & Ish-Horowicz, D 1981, 'Genetic characterization of the region between 86F1,2 and 87B15 on chromosome 3 of Drosophila melanogaster.', Genetics, vol. 98, no. 4, pp. 775-789.
Gausz, J. ; Gyurkovics, H. ; Bencze, G. ; Awad, A. A. ; Holden, J. J. ; Ish-Horowicz, D. / Genetic characterization of the region between 86F1,2 and 87B15 on chromosome 3 of Drosophila melanogaster. In: Genetics. 1981 ; Vol. 98, No. 4. pp. 775-789.
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