Genetic changes of p53, K-ras, and microsatellite instability in gallbladder carcinoma in high-incidence areas of Japan and Hungary

Masayuki Nagahashi, Yoichi Ajioka, Istvan Lang, Zoltan Szentirmay, Miklos Kasler, Hiroto Nakadaira, Naoyuki Yokoyama, Gen Watanabe, Ken Nishikura, Toshifumi Wakai, Yoshio Shirai, Katsuyoshi Hatakeyama, Masaharu Yamamoto

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Abstract

Aim: To disclose geographic differences in genetic changes involved in gallbladder carcinogenesis between two distinct high-incidence areas of Japan and Hungary. Methods: We examined 42 cases of gallbladder carcinoma: 22 Japanese and 20 Hungarian cases. p53 mutations at exons 5 to 8 and K-ras mutations at codon 12 were tested by direct sequencing. Microsatellite instability was determined from fluorescent dye-labeled PCR amplifications of five-microsatellite markers (BAT-25, BAT-26, D2S123, D5S346, and D17S250). Results: Mutations of p53 were detected in 11 of 22 Japanese cases and 6 of 18 Hungarian cases (11/22 vs 6/18, P = 0.348). Transition at CpG sites was found in none of 11 Japanese cases and 2 of 6 Hungarian cases; the difference was marginally significant (0/11 vs 2/6, P = 0.110). K-ras mutations were detected in only one of the Hungarian cases. Eight of 19 (42.1%) Japanese cases were MSI-high (presence of novel peaks in more than one of the five loci analyzed), whereas only 1 of 15 (6.7%) Hungarian cases was MSI-high (P = 0.047). Conclusion: It appears that the p53 mutations and MSI differ in patients with gallbladder carcinoma between two distinct high-incidence areas. Geographic variation might exist in the process of gallbladder carcinogenesis.

Original languageEnglish
Pages (from-to)70-75
Number of pages6
JournalWorld journal of gastroenterology
Volume14
Issue number1
DOIs
Publication statusPublished - Jan 7 2008

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Keywords

  • Gallbladder
  • Gallbladder neoplasms
  • K-ras
  • Microsatellite instability
  • p53

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Nagahashi, M., Ajioka, Y., Lang, I., Szentirmay, Z., Kasler, M., Nakadaira, H., Yokoyama, N., Watanabe, G., Nishikura, K., Wakai, T., Shirai, Y., Hatakeyama, K., & Yamamoto, M. (2008). Genetic changes of p53, K-ras, and microsatellite instability in gallbladder carcinoma in high-incidence areas of Japan and Hungary. World journal of gastroenterology, 14(1), 70-75. https://doi.org/10.3748/wjg.14.70