Genetic and biochemical diversity among Trichoderma isolates in soil samples from winter wheat fields of the great Hungarian plain

L. Kredics, Miklós Láday, Péter Körmöczi, L. Manczinger, G. Rákhely, C. Vágvölgyi, A. Szekeres

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

One hundred and sixteen Trichoderma isolates were collected from chopped roots of winter wheat of five agricultural fields in the Great Hungarian Plain. The isolates were identified by the sequence analysis of the internal transcribed spacer (ITS) region with TrichOKEY 2.0 and BLAST similarity searches. The Trichoderma species detected in the samples were T. atroviride, T. brevicompactum, T. gamsii, T. harzianum, T. koningiopsis/T. ovalisporum, T. longibrachiatum/H. orientalis, T. pleuroticola, T. rossicum, T. spirale, T. tomentosum/T. cerinum and T. virens. Beneficial taxa widely used as biocontrol agents against plant pathogenic fungi (e.g. T. harzianum, T. virens, T. atroviride) could be isolated from the samples examined during this study, indicating that the winter wheat rhizosphere may be a rich source of potential biocontrol isolates. Several species could be characterized with well-defined isoenzyme patterns during cellulose-acetate electrophoresis, suggesting that this method can be used for the analysis of biochemical diversity between and within particular species of the genus Trichoderma.

Original languageEnglish
Pages (from-to)141-149
Number of pages9
JournalActa Biologica Szegediensis
Volume56
Issue number2
Publication statusPublished - 2012

Fingerprint

Biocontrol
Trichoderma
Triticum
winter wheat
Soil
soil sampling
Soils
Electrophoresis
Fungi
Trichoderma atroviride
Cellulose Acetate Electrophoresis
Isoenzymes
cellulose acetate
Rhizosphere
Trichoderma harzianum
plant pathogenic fungi
internal transcribed spacers
electrophoresis
biological control agents
Sequence Analysis

Keywords

  • Biodiversity
  • Trichoderma
  • Winter wheat rhizosphere

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

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title = "Genetic and biochemical diversity among Trichoderma isolates in soil samples from winter wheat fields of the great Hungarian plain",
abstract = "One hundred and sixteen Trichoderma isolates were collected from chopped roots of winter wheat of five agricultural fields in the Great Hungarian Plain. The isolates were identified by the sequence analysis of the internal transcribed spacer (ITS) region with TrichOKEY 2.0 and BLAST similarity searches. The Trichoderma species detected in the samples were T. atroviride, T. brevicompactum, T. gamsii, T. harzianum, T. koningiopsis/T. ovalisporum, T. longibrachiatum/H. orientalis, T. pleuroticola, T. rossicum, T. spirale, T. tomentosum/T. cerinum and T. virens. Beneficial taxa widely used as biocontrol agents against plant pathogenic fungi (e.g. T. harzianum, T. virens, T. atroviride) could be isolated from the samples examined during this study, indicating that the winter wheat rhizosphere may be a rich source of potential biocontrol isolates. Several species could be characterized with well-defined isoenzyme patterns during cellulose-acetate electrophoresis, suggesting that this method can be used for the analysis of biochemical diversity between and within particular species of the genus Trichoderma.",
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T1 - Genetic and biochemical diversity among Trichoderma isolates in soil samples from winter wheat fields of the great Hungarian plain

AU - Kredics, L.

AU - Láday, Miklós

AU - Körmöczi, Péter

AU - Manczinger, L.

AU - Rákhely, G.

AU - Vágvölgyi, C.

AU - Szekeres, A.

PY - 2012

Y1 - 2012

N2 - One hundred and sixteen Trichoderma isolates were collected from chopped roots of winter wheat of five agricultural fields in the Great Hungarian Plain. The isolates were identified by the sequence analysis of the internal transcribed spacer (ITS) region with TrichOKEY 2.0 and BLAST similarity searches. The Trichoderma species detected in the samples were T. atroviride, T. brevicompactum, T. gamsii, T. harzianum, T. koningiopsis/T. ovalisporum, T. longibrachiatum/H. orientalis, T. pleuroticola, T. rossicum, T. spirale, T. tomentosum/T. cerinum and T. virens. Beneficial taxa widely used as biocontrol agents against plant pathogenic fungi (e.g. T. harzianum, T. virens, T. atroviride) could be isolated from the samples examined during this study, indicating that the winter wheat rhizosphere may be a rich source of potential biocontrol isolates. Several species could be characterized with well-defined isoenzyme patterns during cellulose-acetate electrophoresis, suggesting that this method can be used for the analysis of biochemical diversity between and within particular species of the genus Trichoderma.

AB - One hundred and sixteen Trichoderma isolates were collected from chopped roots of winter wheat of five agricultural fields in the Great Hungarian Plain. The isolates were identified by the sequence analysis of the internal transcribed spacer (ITS) region with TrichOKEY 2.0 and BLAST similarity searches. The Trichoderma species detected in the samples were T. atroviride, T. brevicompactum, T. gamsii, T. harzianum, T. koningiopsis/T. ovalisporum, T. longibrachiatum/H. orientalis, T. pleuroticola, T. rossicum, T. spirale, T. tomentosum/T. cerinum and T. virens. Beneficial taxa widely used as biocontrol agents against plant pathogenic fungi (e.g. T. harzianum, T. virens, T. atroviride) could be isolated from the samples examined during this study, indicating that the winter wheat rhizosphere may be a rich source of potential biocontrol isolates. Several species could be characterized with well-defined isoenzyme patterns during cellulose-acetate electrophoresis, suggesting that this method can be used for the analysis of biochemical diversity between and within particular species of the genus Trichoderma.

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