Genetic analysis of two common carp broodstocks by RAPD and microsatellite markers

Richárd Bártfai, Sándor Egedi, Gen Hua Yue, Balázs Kovács, B. Urbányi, Gizella Tamás, László Horváth, László Orbán

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The whole broodstock of two Hungarian common carp farms - 80 and 196 individuals - was analyzed by using random amplified polymorphic DNA (RAPD) assay and microsatellite analysis. Ten polymorphic RAPD markers and four microsatellites were selected to genotype both of the stocks. As expected, microsatellite analysis revealed more detailed information on genetic diversities than RAPD assay. Results obtained with both types of DNA markers showed lack of major differences between the genetic structure of the two stocks: heterozygosity values and allele frequencies were very similar. Dendrograms created from both sets of data did not show grouping of individuals according to stocks. Genotypes from the two stocks were also compared to those from a limited number of samples collected from other hatcheries and two rivers. Allele frequencies in the groups were similar, with the exception of wild carps. An interesting observation was that three private microsatellite alleles were found in the eight wild carp individuals, compared to the seven detected in the rest of the samples tested (372 individuals).

Original languageEnglish
Pages (from-to)157-167
Number of pages11
JournalAquaculture
Volume219
Issue number1-4
DOIs
Publication statusPublished - Apr 2 2003

Fingerprint

random amplified polymorphic DNA technique
breeding stock
genetic analysis
Cyprinus carpio
genetic techniques and protocols
microsatellite repeats
DNA
allele
carp
gene frequency
genotype
assay
genetic markers
assays
hatchery
heterozygosity
genetic structure
hatcheries
farm
alleles

Keywords

  • Cyprinid
  • Cyprinus carpio
  • Fish breeding
  • Genotyping
  • Landrace

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Bártfai, R., Egedi, S., Yue, G. H., Kovács, B., Urbányi, B., Tamás, G., ... Orbán, L. (2003). Genetic analysis of two common carp broodstocks by RAPD and microsatellite markers. Aquaculture, 219(1-4), 157-167. https://doi.org/10.1016/S0044-8486(02)00571-9

Genetic analysis of two common carp broodstocks by RAPD and microsatellite markers. / Bártfai, Richárd; Egedi, Sándor; Yue, Gen Hua; Kovács, Balázs; Urbányi, B.; Tamás, Gizella; Horváth, László; Orbán, László.

In: Aquaculture, Vol. 219, No. 1-4, 02.04.2003, p. 157-167.

Research output: Contribution to journalArticle

Bártfai, R, Egedi, S, Yue, GH, Kovács, B, Urbányi, B, Tamás, G, Horváth, L & Orbán, L 2003, 'Genetic analysis of two common carp broodstocks by RAPD and microsatellite markers', Aquaculture, vol. 219, no. 1-4, pp. 157-167. https://doi.org/10.1016/S0044-8486(02)00571-9
Bártfai, Richárd ; Egedi, Sándor ; Yue, Gen Hua ; Kovács, Balázs ; Urbányi, B. ; Tamás, Gizella ; Horváth, László ; Orbán, László. / Genetic analysis of two common carp broodstocks by RAPD and microsatellite markers. In: Aquaculture. 2003 ; Vol. 219, No. 1-4. pp. 157-167.
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