Genes of the ecdysone biosynthesis pathway are regulated by the dATAC histone acetyltransferase complex in Drosophila

T. Pankotai, C. Popescu, D. Martín, B. Grau, N. Zsindely, L. Bodai, L. Tora, A. Ferrús, I. Boros

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Uncovering mechanisms that regulate ecdysone production is an important step toward understanding the regulation of insect metamorphosis and processes in steroid-related pathologies. We report here the transcriptome analysis of Drosophila melanogaster dAda2a and dAda3 mutants, in which subunits of the ATAC acetyltransferase complex are affected. In agreement with the fact that these mutations lead to lethality at the start of metamorphosis, both the ecdysone levels and the ecdysone receptor binding to polytene chromosomes are reduced in these flies. The cytochrome genes (spookier, phantom, disembodied, and shadow) involved in steroid conversion in the ring gland are downregulated, while the gene shade, which is involved in converting ecdysone into its active form in the periphery, is upregulated in these dATAC subunit mutants. Moreover, driven expression of dAda3 at the site of ecdysone synthesis partially rescues dAda3 mutants. Mutants of dAda2b, a subunit of the dSAGA histone acetyltransferase complex, do not share phenotype characteristics and RNA profile alterations with dAda2a mutants, indicating that the ecdysone biosynthesis genes are regulated by dATAC, but not by dSAGA. Thus, we provide one of the first examples of the coordinated regulation of a functionally linked set of genes by the metazoan-specific ATAC complex.

Original languageEnglish
Pages (from-to)4254-4266
Number of pages13
JournalMolecular and Cellular Biology
Volume30
Issue number17
DOIs
Publication statusPublished - Sep 2010

Fingerprint

Ecdysone
Histone Acetyltransferases
Drosophila
Genes
Steroids
Polytene Chromosomes
Acetyltransferases
Gene Expression Profiling
Cytochromes
Drosophila melanogaster
Diptera
Insects
Down-Regulation
RNA
Pathology
Phenotype
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Genes of the ecdysone biosynthesis pathway are regulated by the dATAC histone acetyltransferase complex in Drosophila. / Pankotai, T.; Popescu, C.; Martín, D.; Grau, B.; Zsindely, N.; Bodai, L.; Tora, L.; Ferrús, A.; Boros, I.

In: Molecular and Cellular Biology, Vol. 30, No. 17, 09.2010, p. 4254-4266.

Research output: Contribution to journalArticle

Pankotai, T. ; Popescu, C. ; Martín, D. ; Grau, B. ; Zsindely, N. ; Bodai, L. ; Tora, L. ; Ferrús, A. ; Boros, I. / Genes of the ecdysone biosynthesis pathway are regulated by the dATAC histone acetyltransferase complex in Drosophila. In: Molecular and Cellular Biology. 2010 ; Vol. 30, No. 17. pp. 4254-4266.
@article{1c0c1a1f51b14f3a811ae6dff76f28c8,
title = "Genes of the ecdysone biosynthesis pathway are regulated by the dATAC histone acetyltransferase complex in Drosophila",
abstract = "Uncovering mechanisms that regulate ecdysone production is an important step toward understanding the regulation of insect metamorphosis and processes in steroid-related pathologies. We report here the transcriptome analysis of Drosophila melanogaster dAda2a and dAda3 mutants, in which subunits of the ATAC acetyltransferase complex are affected. In agreement with the fact that these mutations lead to lethality at the start of metamorphosis, both the ecdysone levels and the ecdysone receptor binding to polytene chromosomes are reduced in these flies. The cytochrome genes (spookier, phantom, disembodied, and shadow) involved in steroid conversion in the ring gland are downregulated, while the gene shade, which is involved in converting ecdysone into its active form in the periphery, is upregulated in these dATAC subunit mutants. Moreover, driven expression of dAda3 at the site of ecdysone synthesis partially rescues dAda3 mutants. Mutants of dAda2b, a subunit of the dSAGA histone acetyltransferase complex, do not share phenotype characteristics and RNA profile alterations with dAda2a mutants, indicating that the ecdysone biosynthesis genes are regulated by dATAC, but not by dSAGA. Thus, we provide one of the first examples of the coordinated regulation of a functionally linked set of genes by the metazoan-specific ATAC complex.",
author = "T. Pankotai and C. Popescu and D. Mart{\'i}n and B. Grau and N. Zsindely and L. Bodai and L. Tora and A. Ferr{\'u}s and I. Boros",
year = "2010",
month = "9",
doi = "10.1128/MCB.00142-10",
language = "English",
volume = "30",
pages = "4254--4266",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "17",

}

TY - JOUR

T1 - Genes of the ecdysone biosynthesis pathway are regulated by the dATAC histone acetyltransferase complex in Drosophila

AU - Pankotai, T.

AU - Popescu, C.

AU - Martín, D.

AU - Grau, B.

AU - Zsindely, N.

AU - Bodai, L.

AU - Tora, L.

AU - Ferrús, A.

AU - Boros, I.

PY - 2010/9

Y1 - 2010/9

N2 - Uncovering mechanisms that regulate ecdysone production is an important step toward understanding the regulation of insect metamorphosis and processes in steroid-related pathologies. We report here the transcriptome analysis of Drosophila melanogaster dAda2a and dAda3 mutants, in which subunits of the ATAC acetyltransferase complex are affected. In agreement with the fact that these mutations lead to lethality at the start of metamorphosis, both the ecdysone levels and the ecdysone receptor binding to polytene chromosomes are reduced in these flies. The cytochrome genes (spookier, phantom, disembodied, and shadow) involved in steroid conversion in the ring gland are downregulated, while the gene shade, which is involved in converting ecdysone into its active form in the periphery, is upregulated in these dATAC subunit mutants. Moreover, driven expression of dAda3 at the site of ecdysone synthesis partially rescues dAda3 mutants. Mutants of dAda2b, a subunit of the dSAGA histone acetyltransferase complex, do not share phenotype characteristics and RNA profile alterations with dAda2a mutants, indicating that the ecdysone biosynthesis genes are regulated by dATAC, but not by dSAGA. Thus, we provide one of the first examples of the coordinated regulation of a functionally linked set of genes by the metazoan-specific ATAC complex.

AB - Uncovering mechanisms that regulate ecdysone production is an important step toward understanding the regulation of insect metamorphosis and processes in steroid-related pathologies. We report here the transcriptome analysis of Drosophila melanogaster dAda2a and dAda3 mutants, in which subunits of the ATAC acetyltransferase complex are affected. In agreement with the fact that these mutations lead to lethality at the start of metamorphosis, both the ecdysone levels and the ecdysone receptor binding to polytene chromosomes are reduced in these flies. The cytochrome genes (spookier, phantom, disembodied, and shadow) involved in steroid conversion in the ring gland are downregulated, while the gene shade, which is involved in converting ecdysone into its active form in the periphery, is upregulated in these dATAC subunit mutants. Moreover, driven expression of dAda3 at the site of ecdysone synthesis partially rescues dAda3 mutants. Mutants of dAda2b, a subunit of the dSAGA histone acetyltransferase complex, do not share phenotype characteristics and RNA profile alterations with dAda2a mutants, indicating that the ecdysone biosynthesis genes are regulated by dATAC, but not by dSAGA. Thus, we provide one of the first examples of the coordinated regulation of a functionally linked set of genes by the metazoan-specific ATAC complex.

UR - http://www.scopus.com/inward/record.url?scp=77956634903&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956634903&partnerID=8YFLogxK

U2 - 10.1128/MCB.00142-10

DO - 10.1128/MCB.00142-10

M3 - Article

C2 - 20584983

AN - SCOPUS:77956634903

VL - 30

SP - 4254

EP - 4266

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 17

ER -