Generation of multidrug resistant human tissues by overexpression of the ABCG2 multidrug transporter in embryonic stem cells

Zsuzsa Erdei, Anita Schamberger, György Török, Kornélia Szebényi, György Várady, Tamás I. Orbán, L. Homolya, B. Sarkadi, A. Apáti

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The ABCG2 multidrug transporter provides resistance against various endo- and xenobiotics, and protects the stem cells against toxins and stress conditions. We have shown earlier that a GFP-tagged version of ABCG2 is fully functional and may be used to follow the expression, localization and function of this transporter in living cells. In the present work we have overexpressed GFP-ABCG2, driven by a constitutive (CAG) promoter, in HUES9 human embryonic stem cells. Stem cell clones were generated to express the wild-type and a substrate-mutant (R482G) GFP-ABCG2 variant, by using the Sleeping Beauty transposon system. We found that the stable overexpression of these transgenes did not change the pluripotency and growth properties of the stem cells, nor their differentiation capacity to hepatocytes or cardiomyocytes. ABCG2 overexpression provided increased toxin resistance in the stem cells, and protected the derived cardiomyocytes against doxorubicin toxicity. These studies document the potential of a stable ABCG2 expression for engineering toxin-resistant human pluripotent stem cells and selected stem cell derived tissues.

Original languageEnglish
Article numbere0194925
JournalPLoS One
Volume13
Issue number4
DOIs
Publication statusPublished - Apr 1 2018

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embryonic stem cells
Embryonic Stem Cells
Stem cells
transporters
stem cells
Stem Cells
Tissue
toxins
Cardiac Myocytes
Beauty
Pluripotent Stem Cells
Xenobiotics
doxorubicin
Transgenes
Doxorubicin
xenobiotics
Hepatocytes
transposons
Clone Cells
hepatocytes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Generation of multidrug resistant human tissues by overexpression of the ABCG2 multidrug transporter in embryonic stem cells. / Erdei, Zsuzsa; Schamberger, Anita; Török, György; Szebényi, Kornélia; Várady, György; Orbán, Tamás I.; Homolya, L.; Sarkadi, B.; Apáti, A.

In: PLoS One, Vol. 13, No. 4, e0194925, 01.04.2018.

Research output: Contribution to journalArticle

Erdei, Zsuzsa ; Schamberger, Anita ; Török, György ; Szebényi, Kornélia ; Várady, György ; Orbán, Tamás I. ; Homolya, L. ; Sarkadi, B. ; Apáti, A. / Generation of multidrug resistant human tissues by overexpression of the ABCG2 multidrug transporter in embryonic stem cells. In: PLoS One. 2018 ; Vol. 13, No. 4.
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