Generating Marker-Free Transgenic Wheat Using Minimal Gene Cassette and Cold-Inducible Cre/Lox System

Klára Mészáros, Csaba Éva, Tibor Kiss, Judit Bányai, Eszter Kiss, Flóra Téglás, L. Láng, I. Karsai, L. Tamás

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The precise elimination of selectable marker genes is highly desirable, when their function is no longer needed, because their presence raised worldwide public concerns against the release of genetically modified plants. This is the first report of simultaneous application of the minimal gene cassette and cold-inducible Cre/lox recombination system in wheat. The bar selection and cre-recombinase genes were eliminated from T0 and T1 transgenic lines with 44 and 51 % efficiency. This approach provides a new, reasonably effective technique to produce selection gene-free transgenic wheat lines either immediately after tissue culture, or from the subsequent transgenic generation. The advantage of this method is that it does not require any additional cold treatment during the plant regeneration/growing because the transgene elimination is ensured by the vernalisation. Application of this method prevents gene flow by pollen and seed, because the selection and recombinase genes are eliminated before pollen development, therefore reducing the risk of GM plants.

Original languageEnglish
Pages (from-to)1221-1231
Number of pages11
JournalPlant Molecular Biology Reporter
Volume33
Issue number5
DOIs
Publication statusPublished - Nov 22 2014

Fingerprint

Triticum
genetically modified organisms
wheat
Genes
Pollen
genes
pollen
vernalization
Recombinases
application methods
Gene Flow
transgenes
Genetically Modified Plants
tissue culture
gene flow
Transgenes
Genetic Recombination
Regeneration
Seeds
genetic markers

Keywords

  • Auto-excision strategy
  • Cold induction
  • Monocot
  • Selectable marker gene
  • Site-specific recombination
  • Wheat

ASJC Scopus subject areas

  • Plant Science
  • Molecular Biology

Cite this

Generating Marker-Free Transgenic Wheat Using Minimal Gene Cassette and Cold-Inducible Cre/Lox System. / Mészáros, Klára; Éva, Csaba; Kiss, Tibor; Bányai, Judit; Kiss, Eszter; Téglás, Flóra; Láng, L.; Karsai, I.; Tamás, L.

In: Plant Molecular Biology Reporter, Vol. 33, No. 5, 22.11.2014, p. 1221-1231.

Research output: Contribution to journalArticle

Mészáros, Klára ; Éva, Csaba ; Kiss, Tibor ; Bányai, Judit ; Kiss, Eszter ; Téglás, Flóra ; Láng, L. ; Karsai, I. ; Tamás, L. / Generating Marker-Free Transgenic Wheat Using Minimal Gene Cassette and Cold-Inducible Cre/Lox System. In: Plant Molecular Biology Reporter. 2014 ; Vol. 33, No. 5. pp. 1221-1231.
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