Generalization of seizures parallels the formation of "dark" neurons in the hippocampus and pontine reticular formation after focal-cortical application of 4-aminopyridine (4-AP) in the rat

Péter Baracskay, Zsuzsanna Szepesi, Gergely Orbán, G. Juhász, A. Czurkó

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Distribution and time course of the occurrence of "dark" neurons were compared with the EEG activity and behavior of rats during 4-aminopyridine (4-AP) induced epileptic seizures. A crystal of the K+ channel blocker 4-AP (0.5 mg/kg) was placed onto the exposed parieto-occipital cortex of Halothane-anesthetized rats for 40 min. Thereafter, the anesthesia was discontinued and the behavioral signs of the epileptic seizure activity were observed. The presence of "dark" neurons was demonstrated by the sensitive silver method of Gallyas in rats sacrificed at 0, 3 and 6 h after the end of the 4-AP crystal application. The EEG activity was recorded in the rats with longer survival times. The EEG analysis revealed the generalization of the epileptic seizures. We found that the formation of "dark" neurons in the hippocampus and the pontine reticular formation paralleled the generalization of the seizures.

Original languageEnglish
Pages (from-to)217-228
Number of pages12
JournalBrain Research
Volume1228
DOIs
Publication statusPublished - Sep 4 2008

Fingerprint

4-Aminopyridine
Hippocampus
Seizures
Neurons
Electroencephalography
Epilepsy
Occipital Lobe
Halothane
Silver
Anesthesia
Pontine Tegmentum

Keywords

  • Animal model
  • Astrocyte
  • Cell injury
  • Dark neuron
  • Epilepsy
  • Hippocampus
  • Neurogliaform cell
  • Pontine reticular formation
  • Status epilepticus

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

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AU - Baracskay, Péter

AU - Szepesi, Zsuzsanna

AU - Orbán, Gergely

AU - Juhász, G.

AU - Czurkó, A.

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N2 - Distribution and time course of the occurrence of "dark" neurons were compared with the EEG activity and behavior of rats during 4-aminopyridine (4-AP) induced epileptic seizures. A crystal of the K+ channel blocker 4-AP (0.5 mg/kg) was placed onto the exposed parieto-occipital cortex of Halothane-anesthetized rats for 40 min. Thereafter, the anesthesia was discontinued and the behavioral signs of the epileptic seizure activity were observed. The presence of "dark" neurons was demonstrated by the sensitive silver method of Gallyas in rats sacrificed at 0, 3 and 6 h after the end of the 4-AP crystal application. The EEG activity was recorded in the rats with longer survival times. The EEG analysis revealed the generalization of the epileptic seizures. We found that the formation of "dark" neurons in the hippocampus and the pontine reticular formation paralleled the generalization of the seizures.

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