Generalised bilayer perturbation from peptide helix dimerisation at membrane surfaces

Vesicle lysis induced by disulphide-dimerised melittin analogues

Jiro Takei, Attila Remenyi, Christopher E. Dempsey

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The effects of covalent dimerisation of melittin by disulphide formation in cysteine-substitution analogues, (melittin K23C)2 and (melittin K23Q,Q25C)2, on the kinetics of pore formation in phosphatidylcholine small unilamellar vesicles was measured under low ionic strength conditions. The initial rate of melittin-induced pore formation increased with the square of the peptide concentration, whereas both disulphide-dimerised melittin analogues showed a first-order dependence of pore formation rates on peptide concentration. These results indicate that peptide dimerisation is rate-limiting for pore formation under these conditions. A model for a generalised bilayer perturbation resulting from the self-association of a pair of peptide helices at the membrane surface is proposed which may have implications for a number of biological processes that involve the interaction of helical polypeptides with membranes. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)11-14
Number of pages4
JournalFEBS Letters
Volume442
Issue number1
DOIs
Publication statusPublished - Jan 8 1999

Fingerprint

Melitten
Dimerization
Disulfides
Membranes
Peptides
Biological Phenomena
Unilamellar Liposomes
Ionic strength
Phosphatidylcholines
Osmolar Concentration
Cysteine
Substitution reactions
Association reactions
Kinetics

Keywords

  • Colicin
  • Magainin
  • Membrane protein
  • Peptide antibiotic
  • Sec-independent

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Generalised bilayer perturbation from peptide helix dimerisation at membrane surfaces : Vesicle lysis induced by disulphide-dimerised melittin analogues. / Takei, Jiro; Remenyi, Attila; Dempsey, Christopher E.

In: FEBS Letters, Vol. 442, No. 1, 08.01.1999, p. 11-14.

Research output: Contribution to journalArticle

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