Gene expression profiling in murine autoimmune arthritis during the initiation and progression of joint inflammation.

Vyacheslav A. Adarichev, C. Vermes, Anita Hanyecz, Katalin Mikecz, Eric G. Bremer, Tibor T. Glant

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We present here an extensive study of differential gene expression in the initiation, acute and chronic phases of murine autoimmune arthritis with the use of high-density oligonucleotide arrays interrogating the entire mouse genome. Arthritis was induced in severe combined immunodeficient mice by using adoptive transfer of lymphocytes from proteoglycan-immunized arthritic BALB/c mice. In this unique system only proteoglycan-specific lymphocytes are transferred from arthritic mice into syngeneic immunodeficient recipients that lack adaptive immunity but have intact innate immunity on an identical (BALB/c) genetic background.Differential gene expression in response to donor lymphocytes that migrated into the joint can therefore be monitored in a precisely timed manner, even before the onset of inflammation. The initiation phase of adoptively transferred disease (several days before the onset of joint swelling) was characterized by differential expression of 37 genes, mostly related to chemokines, interferon-gamma and tumor necrosis factor-alpha signaling, and T cell functions. These were designated early arthritis 'signature' genes because they could distinguish between the naive and the pre-arthritic state. Acute joint inflammation was characterized by at least twofold overexpression of 256 genes and the downregulation of 21 genes, whereas in chronic arthritis a total of 418 genes with an equal proportion of upregulated and downregulated transcripts were expressed differentially.Hierarchical clustering and functional classification of inflammation-related and arthritis-related genes indicated that the most common biological activities were represented by genes encoding interleukins, chemokine receptors and ligands, and by those involved in antigen recognition and processing.

Original languageEnglish
JournalArthritis Research and Therapy
Volume7
Issue number2
Publication statusPublished - 2005

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Gene Expression Profiling
Arthritis
Joints
Inflammation
Genes
Lymphocytes
Proteoglycans
Gene Expression
Down-Regulation
Interleukin Receptors
SCID Mice
Adoptive Transfer
Chemokine Receptors
Antigen Presentation
Adaptive Immunity
Oligonucleotide Array Sequence Analysis
Chemokines
Innate Immunity
Interferon-gamma
Cluster Analysis

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Gene expression profiling in murine autoimmune arthritis during the initiation and progression of joint inflammation. / Adarichev, Vyacheslav A.; Vermes, C.; Hanyecz, Anita; Mikecz, Katalin; Bremer, Eric G.; Glant, Tibor T.

In: Arthritis Research and Therapy, Vol. 7, No. 2, 2005.

Research output: Contribution to journalArticle

Adarichev, Vyacheslav A. ; Vermes, C. ; Hanyecz, Anita ; Mikecz, Katalin ; Bremer, Eric G. ; Glant, Tibor T. / Gene expression profiling in murine autoimmune arthritis during the initiation and progression of joint inflammation. In: Arthritis Research and Therapy. 2005 ; Vol. 7, No. 2.
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