Gene expression profiling identifies FKBP39 as an inhibitor of autophagy in larval Drosophila fat body

G. Juhász, L. Puskás, O. Komonyi, B. Érdi, P. Maróy, T. P. Neufeld, M. Sass

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

In Drosophila, the fat body undergoes a massive burst of autophagy at the end of larval development in preparation for the pupal transition. To identify genes involved in this process, we carried out a microarray analysis. We found that mRNA levels of the homologs of Atg8, the coat protein of early autophagic structures, and lysosomal hydrolases were upregulated, consistent with previous results. Genes encoding mitochondrial proteins and many chaperones were downregulated, including the inhibitor of eIF2alpha kinases and the peptidyl-prolyl cis-trans isomerase FK506-binding protein of 39kDa (FKBP39). Genetic manipulation of FKBP39 expression had a significant effect on autophagy, potentially through modulation of the transcription factor Foxo. Accordingly, we found that Foxo mutants cannot properly undergo autophagy in response to starvation, and that overexpression of Foxo induces autophagy.

Original languageEnglish
Pages (from-to)1181-1190
Number of pages10
JournalCell Death and Differentiation
Volume14
Issue number6
DOIs
Publication statusPublished - Jun 2007

Fingerprint

Tacrolimus Binding Proteins
Fat Body
Autophagy
Gene Expression Profiling
Drosophila
Peptidylprolyl Isomerase
Mitochondrial Proteins
Capsid Proteins
Hydrolases
Microarray Analysis
Starvation
Genes
Transcription Factors
Phosphotransferases
Down-Regulation
Messenger RNA

ASJC Scopus subject areas

  • Cell Biology

Cite this

Gene expression profiling identifies FKBP39 as an inhibitor of autophagy in larval Drosophila fat body. / Juhász, G.; Puskás, L.; Komonyi, O.; Érdi, B.; Maróy, P.; Neufeld, T. P.; Sass, M.

In: Cell Death and Differentiation, Vol. 14, No. 6, 06.2007, p. 1181-1190.

Research output: Contribution to journalArticle

@article{67f53cbbae8a465fab16186d5d8d313f,
title = "Gene expression profiling identifies FKBP39 as an inhibitor of autophagy in larval Drosophila fat body",
abstract = "In Drosophila, the fat body undergoes a massive burst of autophagy at the end of larval development in preparation for the pupal transition. To identify genes involved in this process, we carried out a microarray analysis. We found that mRNA levels of the homologs of Atg8, the coat protein of early autophagic structures, and lysosomal hydrolases were upregulated, consistent with previous results. Genes encoding mitochondrial proteins and many chaperones were downregulated, including the inhibitor of eIF2alpha kinases and the peptidyl-prolyl cis-trans isomerase FK506-binding protein of 39kDa (FKBP39). Genetic manipulation of FKBP39 expression had a significant effect on autophagy, potentially through modulation of the transcription factor Foxo. Accordingly, we found that Foxo mutants cannot properly undergo autophagy in response to starvation, and that overexpression of Foxo induces autophagy.",
author = "G. Juh{\'a}sz and L. Pusk{\'a}s and O. Komonyi and B. {\'E}rdi and P. Mar{\'o}y and Neufeld, {T. P.} and M. Sass",
year = "2007",
month = "6",
doi = "10.1038/sj.cdd.4402123",
language = "English",
volume = "14",
pages = "1181--1190",
journal = "Cell Death and Differentiation",
issn = "1350-9047",
publisher = "Nature Publishing Group",
number = "6",

}

TY - JOUR

T1 - Gene expression profiling identifies FKBP39 as an inhibitor of autophagy in larval Drosophila fat body

AU - Juhász, G.

AU - Puskás, L.

AU - Komonyi, O.

AU - Érdi, B.

AU - Maróy, P.

AU - Neufeld, T. P.

AU - Sass, M.

PY - 2007/6

Y1 - 2007/6

N2 - In Drosophila, the fat body undergoes a massive burst of autophagy at the end of larval development in preparation for the pupal transition. To identify genes involved in this process, we carried out a microarray analysis. We found that mRNA levels of the homologs of Atg8, the coat protein of early autophagic structures, and lysosomal hydrolases were upregulated, consistent with previous results. Genes encoding mitochondrial proteins and many chaperones were downregulated, including the inhibitor of eIF2alpha kinases and the peptidyl-prolyl cis-trans isomerase FK506-binding protein of 39kDa (FKBP39). Genetic manipulation of FKBP39 expression had a significant effect on autophagy, potentially through modulation of the transcription factor Foxo. Accordingly, we found that Foxo mutants cannot properly undergo autophagy in response to starvation, and that overexpression of Foxo induces autophagy.

AB - In Drosophila, the fat body undergoes a massive burst of autophagy at the end of larval development in preparation for the pupal transition. To identify genes involved in this process, we carried out a microarray analysis. We found that mRNA levels of the homologs of Atg8, the coat protein of early autophagic structures, and lysosomal hydrolases were upregulated, consistent with previous results. Genes encoding mitochondrial proteins and many chaperones were downregulated, including the inhibitor of eIF2alpha kinases and the peptidyl-prolyl cis-trans isomerase FK506-binding protein of 39kDa (FKBP39). Genetic manipulation of FKBP39 expression had a significant effect on autophagy, potentially through modulation of the transcription factor Foxo. Accordingly, we found that Foxo mutants cannot properly undergo autophagy in response to starvation, and that overexpression of Foxo induces autophagy.

UR - http://www.scopus.com/inward/record.url?scp=34249096247&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34249096247&partnerID=8YFLogxK

U2 - 10.1038/sj.cdd.4402123

DO - 10.1038/sj.cdd.4402123

M3 - Article

C2 - 17363962

AN - SCOPUS:34249096247

VL - 14

SP - 1181

EP - 1190

JO - Cell Death and Differentiation

JF - Cell Death and Differentiation

SN - 1350-9047

IS - 6

ER -