Gene expression patterns in the bone tissue of women with fibrous dysplasia

János Kiss, Bernadett Balla, János P. Kósa, Adrienn Borsy, János Podani, István Takács, Áron Lazáry, Zsolt Nagy, Krisztián Bácsi, Adrián Kis, Eszter Szlávy, Miklós Szendroi, Gábor Speer, László Orosz, Péter Lakatos

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Fibrous dysplasia is an isolated skeletal disorder caused by a somatic activating mutation of GNAS gene with abnormal unmineralized matrix overproduction and extensive undifferentiated bone cell accumulation in the fibro-osseous lesions. The aim of our investigation was to identify genes that are differently expressed in fibrous versus non-fibrous human bone and to describe the relationships between these genes using multivariate data analysis. Six bone tissue samples from female patients with fibrous dysplastia (FD) and seven bone tissue samples from women without FD (non-FD) were examined. The expression differences of selected 118 genes were analyzed by the TaqMan probe-based quantitative real-time RT-PCR system. The Mann-Whitney U-test indicated marked differences in the expression of 22 genes between FD and non-FD individuals. Nine genes were upregulated in FD women compared to non-FD ones and 18 genes showed a downregulated pattern. These altered genes code for minor collagen molecules, extracellular matrix digesting enzymes, transcription factors, adhesion molecules, growth factors, pro-inflammatory cytokines, and lipid metabolism-affected substrates. Canonical variates analysis demonstrated that FD and non-FD bone tissues can be distinguished by the multiple expression profile analysis of numerous genes controlled via a G-protein coupled pathway and BMP cascade as well as genes coding for extracellular matrix composing molecules. The remarkable changed gene expression profile observed in the fibrous dysplastic human bone tissue may provide further insight into the pathogenetic process of fibrous degeneration of bone.

Original languageEnglish
Pages (from-to)2211-2220
Number of pages10
JournalAmerican Journal of Medical Genetics, Part A
Volume152
Issue number9
DOIs
Publication statusPublished - Sep 1 2010

Keywords

  • Canonical variates analysis
  • Fibrous dysplasia
  • Human subjects
  • Transcriptional profiling

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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