Gene expression analysis of 1,25(OH)2D3-dependent differentiation of HL-60 cells: A cDNA array study

Hakan Savli, Yan Aalto, B. Nagy, Sakari Knuutila, Seppo Pakkala

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The alterations in gene expression associated with 1,25(OH)2D3-induced differentiation of HL-60 cells were studied in order to identify potential targets for further investigation of the genetic basis of acute myeloid leukaemia. Atlas human haematology filters, including 406 genes (Clontech), were used to study gene expression in response to 1,25(OH)2D3 (concentration, 5 × 10-8 mol/l) for 24 and 72 h. Compared with untreated cells, expression differences were found in 43 genes. Downregulated genes at both time-points were: IL2RA, CMYC, NPM, DEK, AF4, FLI1, HTLF, MNDA, BCR, IKAROS, BPI and NFAT4. Upregulated genes at both time-points were IL1B, CD14 and MCL1. CD55, CD58, IRF2, CREB1, ATF4, RAC1, TIAR, KIAA0053, BAT2, BTK, RCK, EV12B and EDN were downregulated at 24 h, while SPI1, MKK3, BTG1 and IL8 were upregulated. At 72 h the upregulated genes were IL1RA, IL2RG, CXCR4, SCYA1, SCYA3, SCYA4, SCYA5, SCYA22, ANX2, CD83 and UPAR. cDNA array results were confirmed on randomly selected genes using quantitative real-time polymerase chain reaction for three upregulated (CXCR4, IL1B and CD14) and three downregulated (DEK, AF4 and FLI1) genes. Gene expression analysis after differentiation induction may provide a tool to study the roles of DEK, AF4 and FLI1 in cell proliferation and differentiation. To demonstrate the genes that initiate differentiation, sequential gene expression analysis has to be performed during the first 24 h of the differentiation process.

Original languageEnglish
Pages (from-to)1065-1070
Number of pages6
JournalBritish Journal of Haematology
Volume118
Issue number4
DOIs
Publication statusPublished - 2002

Fingerprint

HL-60 Cells
Oligonucleotide Array Sequence Analysis
Gene Expression
Genes
Down-Regulation
Atlases
Hematology
Interleukin-8
Acute Myeloid Leukemia
Real-Time Polymerase Chain Reaction
Cell Differentiation
Cell Proliferation

Keywords

  • 1,25(OH)D
  • cDNA array
  • Differentiation
  • Gene expression
  • HL-60

ASJC Scopus subject areas

  • Hematology

Cite this

Gene expression analysis of 1,25(OH)2D3-dependent differentiation of HL-60 cells : A cDNA array study. / Savli, Hakan; Aalto, Yan; Nagy, B.; Knuutila, Sakari; Pakkala, Seppo.

In: British Journal of Haematology, Vol. 118, No. 4, 2002, p. 1065-1070.

Research output: Contribution to journalArticle

Savli, Hakan ; Aalto, Yan ; Nagy, B. ; Knuutila, Sakari ; Pakkala, Seppo. / Gene expression analysis of 1,25(OH)2D3-dependent differentiation of HL-60 cells : A cDNA array study. In: British Journal of Haematology. 2002 ; Vol. 118, No. 4. pp. 1065-1070.
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