Gene and protein expression profiling of the fat-1 mouse brain

Dalma Ménesi, Klára Kitajka, Eszter Molnár, Zoltán Kis, Jérome Belleger, Michael Narce, Jing X. Kang, László G. Puskás, Undurti N. Das

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Polyunsaturated fatty acids (PUFAs) are essential structural components of all cell membranes and, more so, of the central nervous system. Several studies revealed that n-3 PUFAs possess anti-inflammatory actions and are useful in the treatment of dyslipidemia. These actions explain the beneficial actions of n-3 PUFAs in the management of cardiovascular diseases, inflammatory conditions, neuronal dysfunction, and cancer. But, the exact molecular targets of these beneficial actions of n-3 PUFAs are not known. Mice engineered to carry a fat-1 gene from Caenorhabditis elegans add a double bond into an unsaturated fatty acid hydrocarbon chain and convert n-6 to n-3 fatty acids. This results in an abundance of n-3 eicosapentaenoic acid and docosapentaenoic acid specifically in the brain and a reduction in n-6 fatty acids of these mice that can be used to evaluate the actions of n-3 PUFAs. Gene expression profile, RT-PCR and protein microarray studies in the hippocampus and whole brain of wild-type and fat-1 transgenic mice revealed that genes and proteins concerned with inflammation, apoptosis, neurotransmission, and neuronal growth and synapse formation are specifically modulated in fat-1 mice. These results may explain as to why n-3 PUFAs are of benefit in the prevention and treatment of diseases such as Alzheimer's disease, schizophrenia and other diseases associated with neuronal dysfunction, low-grade systemic inflammatory conditions, and bronchial asthma. Based on these data, it is evident that n-3 PUFAs act to modulate specific genes and formation of their protein products and thus, bring about their various beneficial actions.

Original languageEnglish
Pages (from-to)33-42
Number of pages10
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume80
Issue number1
DOIs
Publication statusPublished - Jan 1 2009

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Keywords

  • Alzheimer's disease
  • Fat-1
  • Gene expression
  • Inflammation
  • Neuronal dysfunction
  • Polyunsaturated fatty acids

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

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