Gene and protein expression profiling of the fat-1 mouse brain

Dalma Ménesi, Klára Kitajka, Eszter Molnár, Zoltán Kis, Jérome Belleger, Michael Narce, Jing X. Kang, László G. Puskás, Undurti N. Das

Research output: Contribution to journalArticle

13 Citations (Scopus)


Polyunsaturated fatty acids (PUFAs) are essential structural components of all cell membranes and, more so, of the central nervous system. Several studies revealed that n-3 PUFAs possess anti-inflammatory actions and are useful in the treatment of dyslipidemia. These actions explain the beneficial actions of n-3 PUFAs in the management of cardiovascular diseases, inflammatory conditions, neuronal dysfunction, and cancer. But, the exact molecular targets of these beneficial actions of n-3 PUFAs are not known. Mice engineered to carry a fat-1 gene from Caenorhabditis elegans add a double bond into an unsaturated fatty acid hydrocarbon chain and convert n-6 to n-3 fatty acids. This results in an abundance of n-3 eicosapentaenoic acid and docosapentaenoic acid specifically in the brain and a reduction in n-6 fatty acids of these mice that can be used to evaluate the actions of n-3 PUFAs. Gene expression profile, RT-PCR and protein microarray studies in the hippocampus and whole brain of wild-type and fat-1 transgenic mice revealed that genes and proteins concerned with inflammation, apoptosis, neurotransmission, and neuronal growth and synapse formation are specifically modulated in fat-1 mice. These results may explain as to why n-3 PUFAs are of benefit in the prevention and treatment of diseases such as Alzheimer's disease, schizophrenia and other diseases associated with neuronal dysfunction, low-grade systemic inflammatory conditions, and bronchial asthma. Based on these data, it is evident that n-3 PUFAs act to modulate specific genes and formation of their protein products and thus, bring about their various beneficial actions.

Original languageEnglish
Pages (from-to)33-42
Number of pages10
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Issue number1
Publication statusPublished - Jan 1 2009



  • Alzheimer's disease
  • Fat-1
  • Gene expression
  • Inflammation
  • Neuronal dysfunction
  • Polyunsaturated fatty acids

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

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