GC-MS studies of thiophenes in the supercritical fluid CO2 and solvent extracts of tagetes patula L.

Szabolcs Szarka, István Gyurján, Miklós László, Éva Héthelyi, Inna N. Kuzovkina, Éva Lemberkovics, Éva Szoke

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The intact plant parts and genetically modified hairy root clone #TpA6 of Tagetes patula were extracted with supercritical fluid CO2 extraction (SFE) and a conventional solvent extraction. SFE optimization included the variation of fluid CO2 pressure, dynamic time, and the addition of methanol modifier co-solvent. The four characteristic thiophene metabolites, 5-(3-buten-1-ynyl)-2,2′-bithienyl (BBT), 2,2′:5′,2″- terthiophene (α-T), 5-(4-acetoxy-1-butynyl)-2,2′-bithienyl (BBTOAc), and 5-(3,4-diacetoxy-1-butynyl)-2,2′-bithienyl [BBT(OAc)2], were analysed by GC-MS. The proposed SFE method allowed the selective extraction of thiophenes in 60 min dynamic time with supercritical CO2 without modifier co-solvent, at 30 MPa and 40 °C. The SFE and the reference solvent extraction yielded similar results. The SFE of intact roots and flowers yielded 717 ± 31.3 and 480 ± 26.6 μg g-1 α-T, respectively, while the leaves did not contain considerable amounts of thiophenes. Remarkable amounts of BBT, BBTOAc, and BBT(OAc)2 were characteristic of the SFE of hairy root cultures.

Original languageEnglish
Pages (from-to)1039-1047
Number of pages9
JournalChromatographia
Volume71
Issue number11-12
DOIs
Publication statusPublished - Apr 28 2010

Keywords

  • Acetylenic thiophenes
  • Gas chromatography-mass spectrometry
  • Hairy root culture
  • Supercritical fluid CO extraction
  • Tagetes patula L.

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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