GC-MS of amino acids as their trimethylsilyl/t-butyldimethylsilyl derivatives: In model solutions III

I. Molnár-Perl, Zs F. Katona

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Fragmentation patterns of the essential amino acids (AAs) as their silyl derivatives have been obtained with the aid of ion trap detection (ITD). Three derivatizing reagents, hexamethyldisilazane+trifluoroacetic acid (HMDS+TFAA), bis-(trimethylsilyl)trifluoroacetamide (BSTFA) and N-methyl-N- (t-butyldimethylsilyl)trifluoroacetamide (MTBSTFA) were used. Simple and multiple derivatives obtained with each reagent have been investigated, with regard to their sensitivity and selectivity. Our study performed in the concentration range of 5-2000 ng amino acids has shown that, contrary to literature data, thirteen of the twenty-two AAs investigated including the TBDMS derivatives give rise to more than one peak when eluted. As a result of ion/molecule interaction the very informative ions of high masses, ([M]+, [M+TMS/(TBDMS)]+, [M+1]+) are formed with considerable intensities. The fragments [M-CH3]+, [M-C4H9]+, [M-(CH3)2Si]+, [M-TMS/(TBDMS)COO]+, [M-TBDMSOH]+, [M-TBDMSO]+, [M-TBDMSNH]+ and numerous others could be utilized for identification purposes.

Original languageEnglish
Pages (from-to)S228-S236
JournalChromatographia
Volume51
Issue number1
DOIs
Publication statusPublished - Jan 1 2000

Keywords

  • Amino acids
  • Fragmentation patterns
  • Gaschromatography-mass spectrometry
  • Ion-trap detection
  • Silyl derivatives

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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