GC-MS identification and GC-FID quantitation of terpenoids in Ononidis spinosae Radix

Ágnes E. Daruházi, Szabolcs Szarka, Éva Héthelyi, Béla Simándi, István Gyurján, Miklós László, Éva Szoke, Éva Lemberkovics

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Abstract

A GC-MS method has been developed for the qualitative analysis of sterol and triterpene (terpenoid) constituents of Ononis spinosa L. (spiny restharrow) root without derivatization. β-Sitosterol, campesterol, stigmasterol, stigmastan-3,5-diene sterol compounds and the triterpene derivatives β-amyrin and α-onocerin were identified. A validated GC-FID quantitative method was developed for measuring β-sitosterol, the main sterol component, in various extracts of this plant, obtained with organic solvents and by supercritical fluid extraction. The extracts were cleaned by saponifying and then the β-sitosterol was quantified in the non-saponifiable fractions by GC-FID with an internal standard. In addition, the relative concentrations of the other terpenoids were also determined. The β-sitosterol content in the non-saponifiable solvent extraction fractions was 0.19-5.5%, that of the supercritical fractions were 4.8-9.2%, depending on the experimental conditions. The hexane and the pilot scale SFE extracts were considered as main sources of terpenoids (71.8%; 93.3%, respectively).

Original languageEnglish
Pages (from-to)S71-S76
JournalChromatographia
Volume68
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Oct 1 2008

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Keywords

  • Gas chromatography-flame ionization detection
  • Gas chromatography-mass spectrometry
  • Ononis spinosa L. (spiny restharrow)
  • Supercritical-fluid extraction
  • β-Sitosterol, stigmasterol, campesterol and α-onocerin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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