Gastrin stimulates a cholecystokinin-2-receptor-expressing cardia progenitor cell and promotes progression of Barrett'slike esophagus

Yoomi Lee, Aleksandra M. Urbanska, Yoku Hayakawa, Hongshan Wang, Andrew S. Au, Aesis M. Luna, Wenju Chang, Guangchun Jin, Govind Bhagat, Julian A. Abrams, Richard A. Friedman, A. Varró, Kenneth K. Wang, Malcolm Boyce, Anil K. Rustgi, Antonia R. Sepulveda, Michael Quante, Timothy C. Wang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Objective: The incidence of esophageal adenocarcinoma (EAC) is increasing, but factors contributing to malignant progression of its precursor lesion, Barrett's esophagus (BE), have not been defined. Hypergastrinemia caused by long-term use of proton pump inhibitors (PPIs), has been suggested as one possible risk factor. The gastrin receptor, CCK2R, is expressed in the cardia and upregulated in BE, suggesting the involvement of the gastrin-CCK2R pathway in progression. In the L2-IL-1β mouse model, Barrett's-like esophagus arises from the gastric cardia. Therefore, we aimed to analyze the effect of hypergastrinemia on CCK2R+ progenitor cells in L2-IL-1β mice. Design: L2-IL-1β mice were mated with hypergastrinemic (INS-GAS) mice or treated with PPIs to examine the effect of hypergastrinemia in BE progression. CCK2RCreERT crossed with L2-IL-1β mice were used to analyze the lineage progenitor potential of CCK2R+ cells. Cardia glands were cultured in vitro, and the effect of gastrin treatment analyzed. L2-IL-1β mice were treated with a CCK2R antagonist YF476 as a potential chemopreventive drug. Results: Hypergastrinemia resulted in increased proliferation and expansion of Barrett's-like esophagus. Lineage tracing experiments revealed that CCK2R+ cells are long-lived progenitors that can give rise to such lesions under chronic inflammation. Gastrin stimulated organoid growth in cardia culture, while CCK2R inhibition prevented Barrett's-like esophagus and dysplasia. Conclusions: Our data suggest a progression model for BE to EAC in which CCK2R+ progenitor cells, stimulated by hypergastrinemia, proliferate to give rise to metaplasia and dysplasia. Hypergastrinemia can result from PPI use, and the effects of hypergastrinemia in human BE should be studied further.

Original languageEnglish
Pages (from-to)203-214
Number of pages12
JournalOncotarget
Volume8
Issue number1
DOIs
Publication statusPublished - 2017

Fingerprint

Cholecystokinin B Receptor
Cardia
Barrett Esophagus
Gastrins
Stem Cells
Interleukin-1
Proton Pump Inhibitors
Adenocarcinoma
Organoids
Metaplasia
Stomach
Inflammation
Incidence

Keywords

  • Barrett's esophagus
  • Esophageal cancer
  • Gastrin
  • Gastrin receptors
  • Stem cells

ASJC Scopus subject areas

  • Oncology

Cite this

Gastrin stimulates a cholecystokinin-2-receptor-expressing cardia progenitor cell and promotes progression of Barrett'slike esophagus. / Lee, Yoomi; Urbanska, Aleksandra M.; Hayakawa, Yoku; Wang, Hongshan; Au, Andrew S.; Luna, Aesis M.; Chang, Wenju; Jin, Guangchun; Bhagat, Govind; Abrams, Julian A.; Friedman, Richard A.; Varró, A.; Wang, Kenneth K.; Boyce, Malcolm; Rustgi, Anil K.; Sepulveda, Antonia R.; Quante, Michael; Wang, Timothy C.

In: Oncotarget, Vol. 8, No. 1, 2017, p. 203-214.

Research output: Contribution to journalArticle

Lee, Y, Urbanska, AM, Hayakawa, Y, Wang, H, Au, AS, Luna, AM, Chang, W, Jin, G, Bhagat, G, Abrams, JA, Friedman, RA, Varró, A, Wang, KK, Boyce, M, Rustgi, AK, Sepulveda, AR, Quante, M & Wang, TC 2017, 'Gastrin stimulates a cholecystokinin-2-receptor-expressing cardia progenitor cell and promotes progression of Barrett'slike esophagus', Oncotarget, vol. 8, no. 1, pp. 203-214. https://doi.org/10.18632/oncotarget.10667
Lee, Yoomi ; Urbanska, Aleksandra M. ; Hayakawa, Yoku ; Wang, Hongshan ; Au, Andrew S. ; Luna, Aesis M. ; Chang, Wenju ; Jin, Guangchun ; Bhagat, Govind ; Abrams, Julian A. ; Friedman, Richard A. ; Varró, A. ; Wang, Kenneth K. ; Boyce, Malcolm ; Rustgi, Anil K. ; Sepulveda, Antonia R. ; Quante, Michael ; Wang, Timothy C. / Gastrin stimulates a cholecystokinin-2-receptor-expressing cardia progenitor cell and promotes progression of Barrett'slike esophagus. In: Oncotarget. 2017 ; Vol. 8, No. 1. pp. 203-214.
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abstract = "Objective: The incidence of esophageal adenocarcinoma (EAC) is increasing, but factors contributing to malignant progression of its precursor lesion, Barrett's esophagus (BE), have not been defined. Hypergastrinemia caused by long-term use of proton pump inhibitors (PPIs), has been suggested as one possible risk factor. The gastrin receptor, CCK2R, is expressed in the cardia and upregulated in BE, suggesting the involvement of the gastrin-CCK2R pathway in progression. In the L2-IL-1β mouse model, Barrett's-like esophagus arises from the gastric cardia. Therefore, we aimed to analyze the effect of hypergastrinemia on CCK2R+ progenitor cells in L2-IL-1β mice. Design: L2-IL-1β mice were mated with hypergastrinemic (INS-GAS) mice or treated with PPIs to examine the effect of hypergastrinemia in BE progression. CCK2RCreERT crossed with L2-IL-1β mice were used to analyze the lineage progenitor potential of CCK2R+ cells. Cardia glands were cultured in vitro, and the effect of gastrin treatment analyzed. L2-IL-1β mice were treated with a CCK2R antagonist YF476 as a potential chemopreventive drug. Results: Hypergastrinemia resulted in increased proliferation and expansion of Barrett's-like esophagus. Lineage tracing experiments revealed that CCK2R+ cells are long-lived progenitors that can give rise to such lesions under chronic inflammation. Gastrin stimulated organoid growth in cardia culture, while CCK2R inhibition prevented Barrett's-like esophagus and dysplasia. Conclusions: Our data suggest a progression model for BE to EAC in which CCK2R+ progenitor cells, stimulated by hypergastrinemia, proliferate to give rise to metaplasia and dysplasia. Hypergastrinemia can result from PPI use, and the effects of hypergastrinemia in human BE should be studied further.",
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T1 - Gastrin stimulates a cholecystokinin-2-receptor-expressing cardia progenitor cell and promotes progression of Barrett'slike esophagus

AU - Lee, Yoomi

AU - Urbanska, Aleksandra M.

AU - Hayakawa, Yoku

AU - Wang, Hongshan

AU - Au, Andrew S.

AU - Luna, Aesis M.

AU - Chang, Wenju

AU - Jin, Guangchun

AU - Bhagat, Govind

AU - Abrams, Julian A.

AU - Friedman, Richard A.

AU - Varró, A.

AU - Wang, Kenneth K.

AU - Boyce, Malcolm

AU - Rustgi, Anil K.

AU - Sepulveda, Antonia R.

AU - Quante, Michael

AU - Wang, Timothy C.

PY - 2017

Y1 - 2017

N2 - Objective: The incidence of esophageal adenocarcinoma (EAC) is increasing, but factors contributing to malignant progression of its precursor lesion, Barrett's esophagus (BE), have not been defined. Hypergastrinemia caused by long-term use of proton pump inhibitors (PPIs), has been suggested as one possible risk factor. The gastrin receptor, CCK2R, is expressed in the cardia and upregulated in BE, suggesting the involvement of the gastrin-CCK2R pathway in progression. In the L2-IL-1β mouse model, Barrett's-like esophagus arises from the gastric cardia. Therefore, we aimed to analyze the effect of hypergastrinemia on CCK2R+ progenitor cells in L2-IL-1β mice. Design: L2-IL-1β mice were mated with hypergastrinemic (INS-GAS) mice or treated with PPIs to examine the effect of hypergastrinemia in BE progression. CCK2RCreERT crossed with L2-IL-1β mice were used to analyze the lineage progenitor potential of CCK2R+ cells. Cardia glands were cultured in vitro, and the effect of gastrin treatment analyzed. L2-IL-1β mice were treated with a CCK2R antagonist YF476 as a potential chemopreventive drug. Results: Hypergastrinemia resulted in increased proliferation and expansion of Barrett's-like esophagus. Lineage tracing experiments revealed that CCK2R+ cells are long-lived progenitors that can give rise to such lesions under chronic inflammation. Gastrin stimulated organoid growth in cardia culture, while CCK2R inhibition prevented Barrett's-like esophagus and dysplasia. Conclusions: Our data suggest a progression model for BE to EAC in which CCK2R+ progenitor cells, stimulated by hypergastrinemia, proliferate to give rise to metaplasia and dysplasia. Hypergastrinemia can result from PPI use, and the effects of hypergastrinemia in human BE should be studied further.

AB - Objective: The incidence of esophageal adenocarcinoma (EAC) is increasing, but factors contributing to malignant progression of its precursor lesion, Barrett's esophagus (BE), have not been defined. Hypergastrinemia caused by long-term use of proton pump inhibitors (PPIs), has been suggested as one possible risk factor. The gastrin receptor, CCK2R, is expressed in the cardia and upregulated in BE, suggesting the involvement of the gastrin-CCK2R pathway in progression. In the L2-IL-1β mouse model, Barrett's-like esophagus arises from the gastric cardia. Therefore, we aimed to analyze the effect of hypergastrinemia on CCK2R+ progenitor cells in L2-IL-1β mice. Design: L2-IL-1β mice were mated with hypergastrinemic (INS-GAS) mice or treated with PPIs to examine the effect of hypergastrinemia in BE progression. CCK2RCreERT crossed with L2-IL-1β mice were used to analyze the lineage progenitor potential of CCK2R+ cells. Cardia glands were cultured in vitro, and the effect of gastrin treatment analyzed. L2-IL-1β mice were treated with a CCK2R antagonist YF476 as a potential chemopreventive drug. Results: Hypergastrinemia resulted in increased proliferation and expansion of Barrett's-like esophagus. Lineage tracing experiments revealed that CCK2R+ cells are long-lived progenitors that can give rise to such lesions under chronic inflammation. Gastrin stimulated organoid growth in cardia culture, while CCK2R inhibition prevented Barrett's-like esophagus and dysplasia. Conclusions: Our data suggest a progression model for BE to EAC in which CCK2R+ progenitor cells, stimulated by hypergastrinemia, proliferate to give rise to metaplasia and dysplasia. Hypergastrinemia can result from PPI use, and the effects of hypergastrinemia in human BE should be studied further.

KW - Barrett's esophagus

KW - Esophageal cancer

KW - Gastrin

KW - Gastrin receptors

KW - Stem cells

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