Gastrin radioimmunoassay

Description and application of a novel method

J. Németh, G. Oroszi, B. Jakab, I. Schweibert, J. Szolcsányi, Z. Szilvássy

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In the present paper the development and application of a novel gastrin radioimmunoassay (RIA) are described. 125I-labeling of non-sulphated human gastrin-17 (nshG-17) was performed by the iodogen method and the mono-iodinated hormone, as RIA tracer, was separated by reversed-phase high performance liquid chromatography (HPLC). Serum gastrin levels were measured in response to intravenous application of isoproterenol, a non-selective beta and phenylephrine, a selective alpha-1 receptor agonist using a newly developed method specific for the C-terminal part of the hormone in rats. Isoproterenol at clinically relevant doses elicited a significant increase in serum gastrin concentration in a dose-dependent fashion, whereas phenylephrine was without effect.

Original languageEnglish
Pages (from-to)277-281
Number of pages5
JournalJournal of Radioanalytical and Nuclear Chemistry
Volume254
Issue number2
DOIs
Publication statusPublished - Nov 1 2002

Fingerprint

Gastrins
Radioimmunoassay
Phenylephrine
Isoproterenol
Hormones
High performance liquid chromatography
Reverse-Phase Chromatography
Serum
Labeling
Rats
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Inorganic Chemistry
  • Nuclear Energy and Engineering

Cite this

Gastrin radioimmunoassay : Description and application of a novel method. / Németh, J.; Oroszi, G.; Jakab, B.; Schweibert, I.; Szolcsányi, J.; Szilvássy, Z.

In: Journal of Radioanalytical and Nuclear Chemistry, Vol. 254, No. 2, 01.11.2002, p. 277-281.

Research output: Contribution to journalArticle

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