Gastrin radioimmunoassay: Description and application of a novel method

J. Németh, G. Oroszi, B. Jakab, I. Schweibert, J. Szolcsányi, Z. Szilvássy

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In the present paper the development and application of a novel gastrin radioimmunoassay (RIA) are described. 125I-labeling of non-sulphated human gastrin-17 (nshG-17) was performed by the iodogen method and the mono-iodinated hormone, as RIA tracer, was separated by reversed-phase high performance liquid chromatography (HPLC). Serum gastrin levels were measured in response to intravenous application of isoproterenol, a non-selective beta and phenylephrine, a selective alpha-1 receptor agonist using a newly developed method specific for the C-terminal part of the hormone in rats. Isoproterenol at clinically relevant doses elicited a significant increase in serum gastrin concentration in a dose-dependent fashion, whereas phenylephrine was without effect.

Original languageEnglish
Pages (from-to)277-281
Number of pages5
JournalJournal of Radioanalytical and Nuclear Chemistry
Volume254
Issue number2
DOIs
Publication statusPublished - Nov 1 2002

ASJC Scopus subject areas

  • Analytical Chemistry
  • Nuclear Energy and Engineering
  • Radiology Nuclear Medicine and imaging
  • Pollution
  • Spectroscopy
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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