Gas chromatographic procedure for simultaneous determination of selegiline metabolites, amphetamine, methamphetamine and demethyl-deprenyl in pig plasma

G. Szebeni, J. Lengyel, G. Szekacs, K. Magyar, J. Gaal, I. Szatmari

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A sensitive assay for the simultaneous quantitative determination of amphetamine, methamphetamine and demethyl-deprenyl in pig plasma is described. NP-Gas chromatography is used to determine the extracted plasma concentrations of the three target compounds as their N-penta-fluoro-benzoyl derivatives. Quantitation is performed using 1-phenyl-2-pentylamine as internal standard. The derivatives are separated on a phenyl-methylsilicone capillary column. Quantitation limit for each target compound was 1.2 ng ml-1. Levels of amphetamine, methamphetamine and demethyldeprenyl have been determined in plasma of pigs treated with Selegiline in different formulations and doses.

Original languageEnglish
Pages (from-to)135-141
Number of pages7
JournalActa physiologica Hungarica
Volume83
Issue number2
Publication statusPublished - Jan 1 1995

Keywords

  • GC-NPD procedure
  • amphetamine
  • demethyl-deprenyl
  • methamphetamine
  • selegiline

ASJC Scopus subject areas

  • Physiology (medical)

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