Gas chromatographic method for routine determination of oleanolic and ursolic acids in medicinal plants

G. Janicsák, K. Veres, M. Kállai, I. Máthé

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A rapid and simple gas chromatographic method has been established for routine analysis of free oleanolic and ursolic acids in dried samples of medicinal herbs. Soxhlet extraction of triterpenes was followed by solid-phase extraction (SPE). Amounts of the compounds were measured by gas chromatography after silylation of the purified samples. Experiments were performed to establish the optimum conditions (e.g. solvent, and mode and duration of extraction) for calibration curve linearity, sensitivity, reproducibility, and recovery. The conditions used for derivatization and gas chromatographic analysis resulted in an improvement on literature data. The method devised enables accurate routine measurement of many samples in quite a short time (e.g. for chemotaxonomical screening, or quality control of herbal drugs). The practical application of the method was illustrated on five Lamiaceae species.

Original languageEnglish
Pages (from-to)295-299
Number of pages5
JournalChromatographia
Volume58
Issue number5-6
Publication statusPublished - Sep 2003

Fingerprint

Oleanolic Acid
Medicinal Plants
Gases
Gas chromatography
Gas Chromatography
Lamiaceae
Triterpenes
Solid Phase Extraction
Quality Control
Calibration
Quality control
Screening
Recovery
Pharmaceutical Preparations
ursolic acid
Experiments

Keywords

  • Derivatizatian
  • Gas chromatagraphy
  • Medicinal plants
  • Oleanolic and ursolic acid
  • Solid-phase extraction

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

Gas chromatographic method for routine determination of oleanolic and ursolic acids in medicinal plants. / Janicsák, G.; Veres, K.; Kállai, M.; Máthé, I.

In: Chromatographia, Vol. 58, No. 5-6, 09.2003, p. 295-299.

Research output: Contribution to journalArticle

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