Translated title of the contribution: Gas chromatographic analysis of cannabinoids on 'tandem' columns

G. Petri, S. Nyiredy, P. Veszki, P. Oroszlan, G. Turiak

Research output: Contribution to journalArticle


A simple, isotherm packed column gas chromatographic method was developed for the quantitative determination of neutral cannabinoids using 4-androstene-3,17-dione as internal standard. In order to achieve the best resolution and to avoid the evaluation of the disturbing hydrocarbon peaks a method was developed using 'tandem' column made of 3% OV-1 (90%) and 3% OV-17 (10%) stationary phases. The psychotropic cannabinoids Δ1-tetrahydrocannabinol (Δ1-THC) and Δ1(6)-tetrahydrocannabionol (Δ1(6)-THC), as well as, their main metabolites cannabidiol (CBD) and cannabichromene (CBC) were baseline separated except the cannabigerol (CBG) and cannabinol (CBN) pair, however for these compounds the separation was also satisfactory for the quantitative determinations. The Kovats retention indices were calculated for the most important cannabinoids and established the detection limits, respectively (20-50 ng range). The reproducibility was found excellent cv% = 1.06 for Δ1-THC and the analysis time was 55 minutes. The practical usefulness of the method was demonstrated by the comparative analyses on hashish- and fibre type hemps.

Original languageHungarian
Pages (from-to)63-67
Number of pages5
JournalActa pharmaceutica Hungarica
Issue number3
Publication statusPublished - Jan 1 1995


ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Petri, G., Nyiredy, S., Veszki, P., Oroszlan, P., & Turiak, G. (1995). KANNABINOIDOK GAZKROMATOGRAFIAS VIZSGALATA TANDEM OSZLOPOKON. Acta pharmaceutica Hungarica, 65(3), 63-67.