Gap junctional uncoupling plays a trigger role in the antiarrhythmic effect of ischaemic preconditioning

Rita Papp, Márton Gönczi, Mária Kovács, György Seprényi, A. Végh

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Objective: The aim of this study was to determine whether uncoupling of gap junctions (GJ) prior to ischaemia would modify the antiarrhythmic effect of ischaemic preconditioning (PC) in a canine model of ischaemia/reperfusion. Methods: Twenty control dogs, anaesthetised with chloralose and urethane, were thoracotomised and subjected either to a 25 or a 60 min occlusion of the left anterior descending (LAD) coronary artery. This prolonged ischaemia was preceded 20 min earlier by a single 5 min LAD occlusion in preconditioned dogs (PC group; n = 14) or by a 20 min intracoronary infusion of 50 μM carbenoxolone (CBX group; n = 15), a relatively selective uncoupler of gap junctions. CBX was also infused in PC dogs (CBX + PC group; n = 11). The severity of ischaemia (epicardial ST-segment changes, inhomogeneity of electrical activation) and of ventricular arrhythmias, such as ventricular premature beats (VPBs), ventricular tachycardiac (VT) episodes and ventricular fibrillation (VF), as well as changes in electrical impedance was assessed throughout the experiments. Connexin 43 (Cx43) phosphorylation and GJ permeability were determined at the end of the occlusion periods. Results: Compared to the controls PC and, interestingly, CBX markedly reduced, e.g. the total number of VPBs (440 ± 104 vs 47 ± 11 and 60 ±15; P <0.05) during the prolonged occlusion. This protection was, however, attenuated when CBX was infused in PC dogs (VPBs: 203 ± 32). Changes in electrical impedance, GJ permeability and Cx43 dephosphorylation were significantly less in the PC and CBX groups than in the controls but these were again increased in the CBX + PC group. Conclusions: Uncoupling of GJs prior to ischaemia either by PC or CBX preserves the electrical coupling of cells and results in an antiarrhythmic effect during a subsequent ischaemic insult, indicating that a partial closure of gap junctions may play a trigger role in the protection. In contrast, when CBX is administered in PC dogs the protection both against GJ uncoupling and arrhythmias is markedly attenuated, suggesting that the antiarrhythmic protection, at least in part, is mediated through GJs.

Original languageEnglish
Pages (from-to)396-405
Number of pages10
JournalCardiovascular Research
Volume74
Issue number3
DOIs
Publication statusPublished - Jun 1 2007

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Ischemic Preconditioning
Gap Junctions
Ventricular Premature Complexes
Ischemia
Dogs
Connexin 43
Electric Impedance
Cardiac Arrhythmias
Permeability
Carbenoxolone
Chloralose
Urethane
Ventricular Fibrillation
Reperfusion
Canidae
Coronary Vessels
Phosphorylation
Control Groups

Keywords

  • Connexin 43
  • Electrical impedance
  • Gap junctions
  • Preconditioning
  • Ventricular arrhythmias

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Gap junctional uncoupling plays a trigger role in the antiarrhythmic effect of ischaemic preconditioning. / Papp, Rita; Gönczi, Márton; Kovács, Mária; Seprényi, György; Végh, A.

In: Cardiovascular Research, Vol. 74, No. 3, 01.06.2007, p. 396-405.

Research output: Contribution to journalArticle

Papp, Rita ; Gönczi, Márton ; Kovács, Mária ; Seprényi, György ; Végh, A. / Gap junctional uncoupling plays a trigger role in the antiarrhythmic effect of ischaemic preconditioning. In: Cardiovascular Research. 2007 ; Vol. 74, No. 3. pp. 396-405.
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AU - Végh, A.

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N2 - Objective: The aim of this study was to determine whether uncoupling of gap junctions (GJ) prior to ischaemia would modify the antiarrhythmic effect of ischaemic preconditioning (PC) in a canine model of ischaemia/reperfusion. Methods: Twenty control dogs, anaesthetised with chloralose and urethane, were thoracotomised and subjected either to a 25 or a 60 min occlusion of the left anterior descending (LAD) coronary artery. This prolonged ischaemia was preceded 20 min earlier by a single 5 min LAD occlusion in preconditioned dogs (PC group; n = 14) or by a 20 min intracoronary infusion of 50 μM carbenoxolone (CBX group; n = 15), a relatively selective uncoupler of gap junctions. CBX was also infused in PC dogs (CBX + PC group; n = 11). The severity of ischaemia (epicardial ST-segment changes, inhomogeneity of electrical activation) and of ventricular arrhythmias, such as ventricular premature beats (VPBs), ventricular tachycardiac (VT) episodes and ventricular fibrillation (VF), as well as changes in electrical impedance was assessed throughout the experiments. Connexin 43 (Cx43) phosphorylation and GJ permeability were determined at the end of the occlusion periods. Results: Compared to the controls PC and, interestingly, CBX markedly reduced, e.g. the total number of VPBs (440 ± 104 vs 47 ± 11 and 60 ±15; P <0.05) during the prolonged occlusion. This protection was, however, attenuated when CBX was infused in PC dogs (VPBs: 203 ± 32). Changes in electrical impedance, GJ permeability and Cx43 dephosphorylation were significantly less in the PC and CBX groups than in the controls but these were again increased in the CBX + PC group. Conclusions: Uncoupling of GJs prior to ischaemia either by PC or CBX preserves the electrical coupling of cells and results in an antiarrhythmic effect during a subsequent ischaemic insult, indicating that a partial closure of gap junctions may play a trigger role in the protection. In contrast, when CBX is administered in PC dogs the protection both against GJ uncoupling and arrhythmias is markedly attenuated, suggesting that the antiarrhythmic protection, at least in part, is mediated through GJs.

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