Galactosylated glycan expression and macrophage sensitivity of Lewis lung tumor cells with different metastatic phenotype

J. Tímár, A. Ladányi, K. Lapis, Elemér Moczar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Biochemical and cytochemical analysis of Lewis lung tumor variants revealed that the low metastatic cells contained more galactose/N-acetylgalactosamine residues in a high-molecular-mass (15-20 kDa) mixed N- and O-glycan fraction than the highly metastatic ones. It was also found that the highly metastatic variant was less sensitive to macrophage cytotoxicity in vitro. The cytotoxicity against the low metastatic target cells was inhibited by asialofetuin (10-20 μM), and, to a small degree - and at much higher concentration - by lactose, while galactose and other monosaccharides were ineffective. We suppose that complex galactosylated tumor cell membrane glycans could play a role in the antitumoral cytotoxicity of macrophages.

Original languageEnglish
Pages (from-to)264-270
Number of pages7
JournalJournal of Cancer Research and Clinical Oncology
Volume116
Issue number3
DOIs
Publication statusPublished - May 1990

Fingerprint

Galactose
Polysaccharides
Macrophages
Phenotype
Acetylgalactosamine
Lung
Monosaccharides
Lactose
Neoplasms
Cell Membrane
asialofetuin
In Vitro Techniques

Keywords

  • Galactosylated glycans
  • Macrophage cytotoxicity
  • Metastasis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

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AU - Ladányi, A.

AU - Lapis, K.

AU - Moczar, Elemér

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AB - Biochemical and cytochemical analysis of Lewis lung tumor variants revealed that the low metastatic cells contained more galactose/N-acetylgalactosamine residues in a high-molecular-mass (15-20 kDa) mixed N- and O-glycan fraction than the highly metastatic ones. It was also found that the highly metastatic variant was less sensitive to macrophage cytotoxicity in vitro. The cytotoxicity against the low metastatic target cells was inhibited by asialofetuin (10-20 μM), and, to a small degree - and at much higher concentration - by lactose, while galactose and other monosaccharides were ineffective. We suppose that complex galactosylated tumor cell membrane glycans could play a role in the antitumoral cytotoxicity of macrophages.

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