Gain-of-function screen for genes that affect Drosophila muscle pattern formation

Nicole Staudt, Andreas Molitor, K. Somogyi, Juan Mata, Silvia Curado, Karsten Eulenberg, Martin Meise, Thomas Siegmund, Thomas Häder, Andres Hilfiker, Günter Brönner, Anne Ephrussi, Pernille Rørth, Stephen M. Cohen, Sonja Fellert, Ho Ryun Chung, Olaf Piepenburg, Ulrich Schäfer, Herbert Jäckle, Gerd Vorbrüggen

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

This article reports the production of an EP-element insertion library with more than 3,700 unique target sites within the Drosophila melanogaster genome and its use to systematically identify genes that affect embryonic muscle pattern formation. We designed a UAS/GAL4 system to drive GAL4-responsive expression of the EP-targeted genes in developing apodeme cells to which migrating myotubes finally attach and in an intrasegmental pattern of cells that serve myotubes as a migration substrate on their way towards the apodemes. The results suggest that misexpression of more than 1.5% of the Drosophila genes can interfere with proper myotube guidance and/or muscle attachment. In addition to factors already known to participate in these processes, we identified a number of enzymes that participate in the synthesis or modification of protein carbohydrate side chains and in Ubiquitin modifications and/or the Ubiquitin-dependent degradation of proteins, suggesting that these processes are relevant for muscle pattern formation.

Original languageEnglish
Pages (from-to)499-506
Number of pages8
JournalPLoS Genetics
Volume1
Issue number4
DOIs
Publication statusPublished - 2005

Fingerprint

Skeletal Muscle Fibers
Drosophila
muscle
ubiquitin
Ubiquitin
Muscles
muscles
gene
Body Patterning
Genes
protein
genes
protein degradation
Drosophila melanogaster
transposons
Proteolysis
Libraries
carbohydrate
genome
Carbohydrates

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

Staudt, N., Molitor, A., Somogyi, K., Mata, J., Curado, S., Eulenberg, K., ... Vorbrüggen, G. (2005). Gain-of-function screen for genes that affect Drosophila muscle pattern formation. PLoS Genetics, 1(4), 499-506. https://doi.org/10.1371/journal.pgen.0010055

Gain-of-function screen for genes that affect Drosophila muscle pattern formation. / Staudt, Nicole; Molitor, Andreas; Somogyi, K.; Mata, Juan; Curado, Silvia; Eulenberg, Karsten; Meise, Martin; Siegmund, Thomas; Häder, Thomas; Hilfiker, Andres; Brönner, Günter; Ephrussi, Anne; Rørth, Pernille; Cohen, Stephen M.; Fellert, Sonja; Chung, Ho Ryun; Piepenburg, Olaf; Schäfer, Ulrich; Jäckle, Herbert; Vorbrüggen, Gerd.

In: PLoS Genetics, Vol. 1, No. 4, 2005, p. 499-506.

Research output: Contribution to journalArticle

Staudt, N, Molitor, A, Somogyi, K, Mata, J, Curado, S, Eulenberg, K, Meise, M, Siegmund, T, Häder, T, Hilfiker, A, Brönner, G, Ephrussi, A, Rørth, P, Cohen, SM, Fellert, S, Chung, HR, Piepenburg, O, Schäfer, U, Jäckle, H & Vorbrüggen, G 2005, 'Gain-of-function screen for genes that affect Drosophila muscle pattern formation', PLoS Genetics, vol. 1, no. 4, pp. 499-506. https://doi.org/10.1371/journal.pgen.0010055
Staudt, Nicole ; Molitor, Andreas ; Somogyi, K. ; Mata, Juan ; Curado, Silvia ; Eulenberg, Karsten ; Meise, Martin ; Siegmund, Thomas ; Häder, Thomas ; Hilfiker, Andres ; Brönner, Günter ; Ephrussi, Anne ; Rørth, Pernille ; Cohen, Stephen M. ; Fellert, Sonja ; Chung, Ho Ryun ; Piepenburg, Olaf ; Schäfer, Ulrich ; Jäckle, Herbert ; Vorbrüggen, Gerd. / Gain-of-function screen for genes that affect Drosophila muscle pattern formation. In: PLoS Genetics. 2005 ; Vol. 1, No. 4. pp. 499-506.
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