Gadolinium, a mechano-sensitive channel blocker, inhibits osmosis-initiated motility of sea- and freshwater fish sperm, but does not affect human or ascidian sperm motility

Zoltán Krasznai, Masaaki Morisawa, Zoárd Tibor Krasznai, Sachiko Morisawa, Kazuo Inaba, Zsuzsa Kassai Bazsáné, Bálint Rubovszky, Béla Bodnár, Antal Borsos, Teréz Márián

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Exposure to hypo-osmotic or hyperosmotic environment triggers the initiation of fish sperm motility. In this article, we report that calcium and potassium channel blockers do not influence motility of puffer fish sperm but calmodulin antagonists reversibly decrease it, suggesting that calmodulin-Ca2+ interactions are prerequisite for the initiation of sperm motility in this species. Gadolinium (a stretch activated ion channel blocker) decreased the motility of puffer fish sperm from 92 ± 3% to 6 ± 3% and that of carp sperm from 91 ± 7% to 3.5 ± 4.3% in a dose-dependent manner (10-40 μM). The effect of gadolinium was reversible, suggesting that stretch activated ion channels participate in the initiation of sperm motility of the two species. Gadolinium inhibits changes in the isoelectric point of certain proteins of puffer fish sperm, which occur when sperm motility is initiated in a hypertonic solution. Anisotropy measurements showed that hypo-osmotic treatment, which initiates carp sperm motility, increased membrane fluidity. When hypo-osmotic treatment was given in the presence of gadolinium, the sperm membrane remained as rigid as in quiescent cells, while motility was blocked. By contrast, gadolinium did not influence the motility parameters of Ciona or human sperm. Based on these lines of evidence, we suggest that conformational changes of mechanosensitive membrane proteins are involved in osmolality-dependent but not osmolality-independent sperm.

Original languageEnglish
Pages (from-to)232-243
Number of pages12
JournalCell Motility and the Cytoskeleton
Volume55
Issue number4
DOIs
Publication statusPublished - Aug 1 2003

Keywords

  • Fish sperm
  • Gadolinium
  • Human sperm
  • Sperm motility and membrane fluidity
  • Stretch activated channels

ASJC Scopus subject areas

  • Structural Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Gadolinium, a mechano-sensitive channel blocker, inhibits osmosis-initiated motility of sea- and freshwater fish sperm, but does not affect human or ascidian sperm motility'. Together they form a unique fingerprint.

  • Cite this