GAD isoforms exhibit distinct spatiotemporal expression patterns in the developing mouse lens: Correlation with Dlx2 and Dlx5

Andrea Kwakowsky, Marija Schwirtlich, Qi Zhang, David D. Eisenstat, Ferenc Erdélyi, Maria Baranyi, Zoya D. Katarova, Gábor Szabó

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Gamma-aminobutyric acid (GABA), the major inhibitory neurotransmitter of the adult nervous system and its biosynthetic enzyme glutamic acid decarboxylase (GAD) are abundantly expressed in the embryonic nervous system and are involved in the modulation of cell proliferation, migration, and differentiation. Here we describe for the first time the expression of GABA and embryonic and adult GAD isoforms in the developing mouse lens. We show that the GAD isoforms are sequentially induced with specific spatiotemporal profiles: GAD65 and embryonic GAD isoforms prevail in primary fibers, while GAD67 is the predominant GAD expressed in the postnatal secondary fibers. This pattern correlates well with the expression of Dlx2 and Dlx5, known as upstream regulators of GAD. GABA and GAD are most abundant at the tips of elongating fibers and are absent from organelle-free cells, suggesting their involvement is primarily in shaping of the cytoskeleton during fiber elongation stages.

Original languageEnglish
Pages (from-to)3532-3544
Number of pages13
JournalDevelopmental Dynamics
Volume236
Issue number12
DOIs
Publication statusPublished - Dec 2007

Fingerprint

Glutamate Decarboxylase
Lenses
Protein Isoforms
gamma-Aminobutyric Acid
Nervous System
Cytoskeleton
Organelles
Cell Movement
Neurotransmitter Agents
Cell Differentiation
Cell Proliferation
Enzymes

Keywords

  • Dlx
  • Embryonic GAD
  • GABA
  • GAD65
  • GAD65/gfp transgenic mice
  • GAD67
  • Lens fiber cells
  • Prox1

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

GAD isoforms exhibit distinct spatiotemporal expression patterns in the developing mouse lens : Correlation with Dlx2 and Dlx5. / Kwakowsky, Andrea; Schwirtlich, Marija; Zhang, Qi; Eisenstat, David D.; Erdélyi, Ferenc; Baranyi, Maria; Katarova, Zoya D.; Szabó, Gábor.

In: Developmental Dynamics, Vol. 236, No. 12, 12.2007, p. 3532-3544.

Research output: Contribution to journalArticle

Kwakowsky, Andrea ; Schwirtlich, Marija ; Zhang, Qi ; Eisenstat, David D. ; Erdélyi, Ferenc ; Baranyi, Maria ; Katarova, Zoya D. ; Szabó, Gábor. / GAD isoforms exhibit distinct spatiotemporal expression patterns in the developing mouse lens : Correlation with Dlx2 and Dlx5. In: Developmental Dynamics. 2007 ; Vol. 236, No. 12. pp. 3532-3544.
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