GABAA receptor-controlled 36Cl- influx in cultured rat cerebellar granule cells

Julianna Kardos, Katalin Maderspach

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

γ{phonetic}-Aminobutyric acid (GABA)-mediated and bicuculline-sensitive 36Cl- influx and bicuculline-sensitive [3H] GABA binding were demonstrated in cultures of rat cerebellar granule cells. The addition of 10-5 M GABA produced a two-fold increase in 36Cl- influx over the basal level and the maximal increase was observed after approximately 20 sec. Progressive occupation of GABAA receptor by [3H]-(1S-9R)-bicuculline methiodide decreased 36Cl- influx activated by 10 μM GABA. The above results suggest that primary cultures of rat cerebellar granule cells provide a new and reliable model for studying the GABA activated chloride fluxes.

Original languageEnglish
Pages (from-to)265-272
Number of pages8
JournalLife Sciences
Volume41
Issue number3
DOIs
Publication statusPublished - Jul 20 1987

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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