GABAA receptor-controlled 36Cl- influx in cultured rat cerebellar granule cells

J. Kardos, Katalin Maderspach

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

γ{phonetic}-Aminobutyric acid (GABA)-mediated and bicuculline-sensitive 36Cl- influx and bicuculline-sensitive [3H] GABA binding were demonstrated in cultures of rat cerebellar granule cells. The addition of 10-5 M GABA produced a two-fold increase in 36Cl- influx over the basal level and the maximal increase was observed after approximately 20 sec. Progressive occupation of GABAA receptor by [3H]-(1S-9R)-bicuculline methiodide decreased 36Cl- influx activated by 10 μM GABA. The above results suggest that primary cultures of rat cerebellar granule cells provide a new and reliable model for studying the GABA activated chloride fluxes.

Original languageEnglish
Pages (from-to)265-272
Number of pages8
JournalLife Sciences
Volume41
Issue number3
DOIs
Publication statusPublished - Jul 20 1987

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GABA-A Receptors
gamma-Aminobutyric Acid
Rats
Bicuculline
Aminobutyrates
Phonetics
Speech analysis
Occupations
Chlorides
Fluxes

ASJC Scopus subject areas

  • Pharmacology

Cite this

GABAA receptor-controlled 36Cl- influx in cultured rat cerebellar granule cells. / Kardos, J.; Maderspach, Katalin.

In: Life Sciences, Vol. 41, No. 3, 20.07.1987, p. 265-272.

Research output: Contribution to journalArticle

Kardos, J. ; Maderspach, Katalin. / GABAA receptor-controlled 36Cl- influx in cultured rat cerebellar granule cells. In: Life Sciences. 1987 ; Vol. 41, No. 3. pp. 265-272.
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