GABAergic phenotypic differentiation of a subpopulation of subventricular derived migrating progenitors

S. De Marchis, S. Temoney, F. Erdelyi, S. Bovetti, P. Bovolin, G. Szabo, A. C. Puche

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Olfactory bulb interneurons are continuously generated throughout development and in adulthood. These neurons are born in the subventricular zone (SVZ) and migrate along the rostral migratory stream into the olfactory bulb where they differentiate into local interneurons. To investigate the differentiation of GABAergic interneurons of the olfactory bulb we used a transgenic mouse which expresses green fluorescent protein (GFP) under the control of the glutamic acid decarboxylase 65 KDa (GAD65) promoter. During development and in adulthood GFP was expressed by cells in the SVZ and along the entire length of its rostral extension including the distal portion within the olfactory bulb. The occurrence of GAD65 mRNA in these zones was confirmed by PCR analysis on microdissected regions along the pathway. Polysialic acid neural cell adhesion molecule, a marker of migrating neuroblasts in adults, was coexpressed by the majority of the GFP-positive SVZ-derived progenitor cells. Cell tracer injections into the SVZ indicated that ≈26% of migrating progenitor cells expressed GFP. These data show the early differentiation of migrating SVZ-derived progenitors into a GAD65-GFP-positive phenotype. These cells could represent a restricted lineage giving rise to GAD65-positive GABAergic olfactory bulb interneurons.

Original languageEnglish
Pages (from-to)1307-1317
Number of pages11
JournalEuropean Journal of Neuroscience
Volume20
Issue number5
DOIs
Publication statusPublished - Sep 1 2004

Keywords

  • Differentiation
  • Lineage
  • Migration
  • Olfactory bulb
  • Rostral migratory stream
  • Stem cell

ASJC Scopus subject areas

  • Neuroscience(all)

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