GABA regulates dendritic growth by stabilizing lamellipodia in newly generated interneurons of the olfactory bulb

Eduardo Gascon, Alexandre G. Dayer, Marc Olivier Sauvain, Gael Potter, Benoit Jenny, Mathias De Roo, Eloisa Zgraggen, Nicolas Demaurex, Dominique Muller, J. Kiss

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The initial formation and growth of dendrites is a critical step leading to the integration of newly generated neurons into postnatal functional networks. However, the cellular mechanisms and extracellular signals regulating this process remain mostly unknown. By directly observing newborn neurons derived from the subventricular zone in culture as well as in olfactory bulb slices, we show that ambient GABA acting through GABAA receptors is essential for the temporal stability of lamellipodial protrusions in dendritic growth cones but did not interfere with filopodia dynamics. Furthermore, we provide direct evidence that ambient GABA is required for the proper initiation and elongation of dendrites by promoting the rapid stabilization of new dendritic segments after their extension. The effects of GABA on the initial formation of dendrites depend on depolarization and Ca2+ influx and are associated with a higher stability of microtubules. Together, our results indicate that ambient GABA is a key regulator of dendritic initiation in postnatally generated olfactory interneurons and offer a mechanism by which this neurotransmitter drives early dendritic growth.

Original languageEnglish
Pages (from-to)12956-12966
Number of pages11
JournalJournal of Neuroscience
Volume26
Issue number50
DOIs
Publication statusPublished - Dec 13 2006

Fingerprint

Pseudopodia
Olfactory Bulb
Interneurons
gamma-Aminobutyric Acid
Dendrites
Growth
Neurons
Growth Cones
Lateral Ventricles
GABA-A Receptors
Microtubules
Neurotransmitter Agents

Keywords

  • Dendritic development
  • GABA
  • Lamellipodia
  • Microtubule
  • Neurogenesis
  • Subventricular zone

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

GABA regulates dendritic growth by stabilizing lamellipodia in newly generated interneurons of the olfactory bulb. / Gascon, Eduardo; Dayer, Alexandre G.; Sauvain, Marc Olivier; Potter, Gael; Jenny, Benoit; De Roo, Mathias; Zgraggen, Eloisa; Demaurex, Nicolas; Muller, Dominique; Kiss, J.

In: Journal of Neuroscience, Vol. 26, No. 50, 13.12.2006, p. 12956-12966.

Research output: Contribution to journalArticle

Gascon, E, Dayer, AG, Sauvain, MO, Potter, G, Jenny, B, De Roo, M, Zgraggen, E, Demaurex, N, Muller, D & Kiss, J 2006, 'GABA regulates dendritic growth by stabilizing lamellipodia in newly generated interneurons of the olfactory bulb', Journal of Neuroscience, vol. 26, no. 50, pp. 12956-12966. https://doi.org/10.1523/JNEUROSCI.4508-06.2006
Gascon, Eduardo ; Dayer, Alexandre G. ; Sauvain, Marc Olivier ; Potter, Gael ; Jenny, Benoit ; De Roo, Mathias ; Zgraggen, Eloisa ; Demaurex, Nicolas ; Muller, Dominique ; Kiss, J. / GABA regulates dendritic growth by stabilizing lamellipodia in newly generated interneurons of the olfactory bulb. In: Journal of Neuroscience. 2006 ; Vol. 26, No. 50. pp. 12956-12966.
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