G-protein coupled activation of potassium channels by endogenous neuropeptides in snail neurons

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Members of the mytilus inhibitory peptide (MIP) family play an important role in the modulation of many physiological processes in molluscs. The signal transduction pathways affected by the MIP effect have not, however, been elucidated.Application of guanosine 5′-[γ-thio]triphosphate tetralithium salt (GTPγS), guanosine 5′-[β-thio]diphosphate trilithium salt (GDPβS), the G-protein inhibitor suramin and pertussis toxin (PTX) demonstrated the involvement of the PTX-insensitive G-protein in the signal transduction pathway mediating MIP effects. Both G-protein αi and βγ subunits were identified in D-neurons of Helix pomatia by immunoblotting. Their role in signal transduction was shown in electrophysiological experiments, which supported the notion that, in addition to the Gα subunit, the βγ dimer also participates in the neuropeptide-induced activation of K-channels in snail neurons. Finally, neuropeptide-activated responses were inhibited by the activation of adenylyl cyclase and by blockers of the phospholipase pathway. We suggest that bifurcation of the signal transduction takes place at the level of G-protein subunits. The α subunit may have a direct effect on adenylyl cyclase, while the βγ subunit may have a direct effect on phospholipase enzymes.

Original languageEnglish
Pages (from-to)2177-2185
Number of pages9
JournalEuropean Journal of Neuroscience
Volume21
Issue number8
DOIs
Publication statusPublished - Apr 2005

Fingerprint

Potassium Channels
Snails
Neuropeptides
GTP-Binding Proteins
Signal Transduction
Neurons
Phospholipases
Guanosine
Pertussis Toxin
Protein Subunits
Adenylyl Cyclases
Salts
Physiological Phenomena
Suramin
Diphosphates
Mollusca
Immunoblotting
Enzymes
Mytilus inhibitory peptide

Keywords

  • α subunit
  • βγ subunit
  • cAMP
  • G-protein
  • K-channel
  • Phospholipase

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

G-protein coupled activation of potassium channels by endogenous neuropeptides in snail neurons. / Kiss, T.

In: European Journal of Neuroscience, Vol. 21, No. 8, 04.2005, p. 2177-2185.

Research output: Contribution to journalArticle

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