The investigation of chromatographic (HPLC) systems containing camphorsulfonic acid (CSA) has been continued. Similarly to the previous finding for silica, on polar bonded silica packings CSA forms double layer. The adsorbed amount of CSA was determined by the evaluation of break-through curves. It was found that the addition of triethylamine in a small excess to the CSA containing eluent results in the formation of a versatile chromatographic system. The adsorbed amount of CSA and TEA from such eluents was determined by the estimation of the break-through curves were gained by simultaneous refractometric and UV spectrophotometric detection. The migration of the compounds follows a normal phase mechanism evidenced by the behavior of the examined medicinal compounds.
ASJC Scopus subject areas
- Molecular Medicine