Further evidence that the vitopine-type pTi's of Agrobacterium vitis Represent a novel group of Ti plasmids

E. Szegedi, Mihály Czakó, Léon Otten

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

To study the incompatibility properties of the vitopine Ti plasmids of Agrobacterium vitis, pPM739 containing the cloned ori region of pTiS4 was introduced by triparental mating into seven Agrobacterium tumefaciens strains carrying incRh-1, incRh-2, and incAg-1 plasmids and into eight A. vitis strains. All strains containing pPM739 retained their original plasmids and virulence or the ability to grow on tartrate, except for the three vitopine strains S4, Sz1, and NW11. Furthermore, pTiS4 was stably maintained in S4 cells following introduction of the ori/inc clones of the incRh-1, incRh-2, and incRh-3 plasmids. These results show that vitopine Ti plasmids represent a novel incompatibility group for which we propose the name incRh-4.

Original languageEnglish
Pages (from-to)139-143
Number of pages5
JournalMolecular Plant-Microbe Interactions
Volume9
Issue number2
Publication statusPublished - Mar 1996

Fingerprint

Plant Tumor-Inducing Plasmids
Rhizobium vitis
Agrobacterium
Vitis
plasmids
Plasmids
Agrobacterium tumefaciens
Replication Origin
Names
Virulence
replication origin
Clone Cells
Agrobacterium radiobacter
virulence
vitopine
clones

Keywords

  • Crown gall
  • Grapevine
  • Opines
  • Plasmid incompatibility

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Microbiology

Cite this

Further evidence that the vitopine-type pTi's of Agrobacterium vitis Represent a novel group of Ti plasmids. / Szegedi, E.; Czakó, Mihály; Otten, Léon.

In: Molecular Plant-Microbe Interactions, Vol. 9, No. 2, 03.1996, p. 139-143.

Research output: Contribution to journalArticle

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AB - To study the incompatibility properties of the vitopine Ti plasmids of Agrobacterium vitis, pPM739 containing the cloned ori region of pTiS4 was introduced by triparental mating into seven Agrobacterium tumefaciens strains carrying incRh-1, incRh-2, and incAg-1 plasmids and into eight A. vitis strains. All strains containing pPM739 retained their original plasmids and virulence or the ability to grow on tartrate, except for the three vitopine strains S4, Sz1, and NW11. Furthermore, pTiS4 was stably maintained in S4 cells following introduction of the ori/inc clones of the incRh-1, incRh-2, and incRh-3 plasmids. These results show that vitopine Ti plasmids represent a novel incompatibility group for which we propose the name incRh-4.

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