Fungal biosynthesis of endochitinase and chitobiase in solid state fermentation and their application for the production of N-acetyl-d-glucosamine from colloidal chitin

Parameswaran Binod, Chandran Sandhya, Pradeep Suma, G. Szakács, Ashok Pandey

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The present study was directed to the production of N-acetyl-d-glucosamine using endochitinase and chitobiase from fungal cultures in solid culturing. Fifteen fungal strains were evaluated for endochitinase and chitobiase production under solid-state fermentation using agro-industrial residues, of which Penicillium aculeatum NRRL 2129 showed maximum endochitinase activity whereas Trichoderma harzianum TUBF 927 showed maximum chitobiase activity. Eleven substrates, alone and in combination with chitin, were evaluated for the enzyme production. Optimization of physico-chemical parameters such as incubation period and initial moisture content, and nutritional parameters such as chitin source, inorganic and organic nitrogen sources, were carried out. Optimization resulted in more than 3-fold increase in endochitinase production (from 3.5 to 12.53 U/g dry weight of substrate) and about 1.5-fold increase in chitobiase production (from 1.6 to 2.25 U/g dry weight of substrate). Studies on the degradation of colloidal chitin to N-acetyl-d-glucosamine showed improved efficiency when endochitinase and chitobiase were used in combination.

Original languageEnglish
Pages (from-to)2742-2748
Number of pages7
JournalBioresource Technology
Volume98
Issue number14
DOIs
Publication statusPublished - Oct 2007

Fingerprint

Glucosamine
beta-N-acetylhexosaminidase
Acetylglucosaminidase
Chitinases
Chitin
solid state fermentation
chitin
glucosamine
Biosynthesis
Fermentation
fermentation
biosynthesis
substrate
fold
inorganic nitrogen
organic nitrogen
Substrates
moisture content
Weights and Measures
incubation

Keywords

  • Chitobiase
  • Colloidal chitin
  • Degradation
  • Endochitinase
  • N-Acetyl-d-glucosamine
  • Production
  • Solid-state fermentation

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Process Chemistry and Technology
  • Applied Microbiology and Biotechnology

Cite this

Fungal biosynthesis of endochitinase and chitobiase in solid state fermentation and their application for the production of N-acetyl-d-glucosamine from colloidal chitin. / Binod, Parameswaran; Sandhya, Chandran; Suma, Pradeep; Szakács, G.; Pandey, Ashok.

In: Bioresource Technology, Vol. 98, No. 14, 10.2007, p. 2742-2748.

Research output: Contribution to journalArticle

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