Functioning of the bidirectional hydrogenase in different unicellular cyanobacteria

Éva Kiss, Péter B. Kós, Min Chen, Imre Vass

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Synechocystis PCC 6083 (Synechocystis) cells produce hydrogen in the absence of light, during oxygen deprivation. The enzyme responsible for the hydrogen production in this strain is the hox hydrogenase, which catalyses the simple H2↔2H++2e redox reaction. This bidirectional hydrogenase is also found in Synechococcus elongatus PCC 7942 (Synechococcus) and Acaryochloris marina (Acaryochloris). However, we could not detect in vivo hydrogen production neither in Synechococcus nor in Acaryochloris. In Synechocystis, in a dark, hypoxic environment a strongly reduced plastoquinon (PQ) pool implies that there are excess electrons and protons available for the hydrogenase, which is present in the cell in an elevated level shown by RT-PCR. Conversely, we found that the hox genes are suppressed in Synechococcus under the same conditions. In Acaryochloris a dark hypoxic environment induces the hox genes. Despite the elevated enzyme level in Acaryochloris H2 production is undetectable, likely because of the shortage of reducing equivalents available for the hydrogenase that is indicated by a feeble PQ pool reduction.

Original languageEnglish
Title of host publicationAdvanced Topics in Science and Technology in China
PublisherSpringer
Pages733-736
Number of pages4
DOIs
Publication statusPublished - Jan 1 2013

Publication series

NameAdvanced Topics in Science and Technology in China
ISSN (Print)1995-6819
ISSN (Electronic)1995-6827

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Keywords

  • Acaryochloris marina
  • Hox hydrogenase
  • Synechococcus elongatus 7942
  • Synechocystis 6803

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Engineering(all)
  • General

Cite this

Kiss, É., Kós, P. B., Chen, M., & Vass, I. (2013). Functioning of the bidirectional hydrogenase in different unicellular cyanobacteria. In Advanced Topics in Science and Technology in China (pp. 733-736). (Advanced Topics in Science and Technology in China). Springer. https://doi.org/10.1007/978-3-642-32034-7_157