Functionally and morphologically distinct populations of extracellular vesicles produced by human neutrophilic granulocytes

Ákos M. Lőrincz, Maria Schütte, Csaba I. Timár, Daniel S. Veres, Ágnes Kittel, Kenneth R. McLeish, Michael L. Merchant, Erzsébet Ligeti

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

EVs in the microvesicle size range released during spontaneous death of human neutrophils were charac- terized and their properties compared with previously described EVs with antibacterial effect (aEVs, generated on specific activation) or produced spontaneously (sEVs). The 3 vesicle populations overlapped in size and in part of the constituent proteins were stained with annexin V and were impermeable to PI. However, none of them produced superoxide. In contrast, remarkable differences were observed in the morphology, abun- dance of proteins, and antibacterial function. EVs formed spontaneously in 30 min (sEVs) were more similar to EVs released during spontaneous death in 1-3 d than to EVs formed in 30 min on stimulation of opsonin receptors (aEVs). Spontaneously generated EVs had no antibacterial effect despite their large number and protein content. We hypothesized 2 parallel mechanisms: one that proceeds spontaneously and produces EVs without antibacterial effect and another process that is triggered by opsonin receptors and results in differential sorting of proteins into EVs with antibacterial capacity. Our results call attention to the functional and morphologic heterogeneity within the microvesicle/ectosome fraction of EVs.

Original languageEnglish
Pages (from-to)583-589
Number of pages7
JournalJournal of Leukocyte Biology
Volume98
Issue number4
DOIs
Publication statusPublished - Oct 2015

Keywords

  • Apoptotic bodies
  • Bacterial survival
  • Microvesicles
  • Protein profile
  • Spontaneous death

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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